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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
-20°C
- 克隆性:
Polyclonal
- 标记物:
Biotin
- 适应物种:
Rabbit
- 保质期:
12 months
- 级别:
二抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Goat
- 应用范围:
WB;IHC;ELISA
- 抗体英文名:
Biotin-Goat Anti-Rabbit IgG F(ab')2
- 规格:
120μL/500μL
| 规格: | 120μL | 产品价格: | ¥160.0 |
|---|---|---|---|
| 规格: | 500μL | 产品价格: | ¥640.0 |
| 产品名称 | Biotin-Goat Anti-Rabbit IgG F(ab')2 |
| 产品货号 | E-AB-1114 |
| 宿主 | Goat |
| 反应性 | Rabbit |
| 应用性 | WB;IHC;ELISA |
| 偶联物 | Biotin |
| 亚型 | IgG |
| 克隆性 | Polyclonal |
| 运输条件 | Ice bag |
| 保存及保质期 | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |

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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
Ab concentrations.OD (Absorbance at 280 nm)/1.35 = X mg/ml.Store Abs at -20℃ or further concentrate by using Centriprep. And store at -20℃.Regenerate Ab column with Regenerate buffer (~200 ml).
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
cut paraffin sections and deparaffinize with xylene and graded EtOH into PBS. Block slides with 1/20x serum/PBS (use the appropriate blocking serum, i.e. same as 1º Ab), 10'. Wash in PBS 2' x3. Add digoxigenin labeled 1º antiobody.
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