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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 克隆性:
Monoclonal
- 标记物:
FITC;PE;PerCP/Cyanine 5.5
- 适应物种:
Human
- 保质期:
12 months
- 级别:
流式抗体
- 供应商:
武汉益普生物科技有限公司
- 应用范围:
FCM
- 抗体英文名:
Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5 Cocktail
- 规格:
20Tests/100Tests/100Tests×2
| 规格: | 20Tests | 产品价格: | ¥1020.0 |
|---|---|---|---|
| 规格: | 100Tests | 产品价格: | ¥2580.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥4380.0 |
| 产品名称 | Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5 Cocktail |
| 产品货号 | E-AB-FC0003 |
| 反应性 | Human |
| 应用性 | FCM |
| 偶联物 | FITC;PE;PerCP/Cyanine 5.5 |
| 克隆性 | Monoclonal |
| 克隆号 | OKT-3;RPA-T4;HI30 |
| 运输条件 | 冰袋 |
| 保存及保质期 | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |

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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
on the laboratory preference): 1xPBS or 50mM Tris 7.4/ 150mM NaCl supplemented with 1% NP40. 0.25% Na-deoxycholate. 1mM EDTA with 1mM PMSF and protease inhibitor cocktail. 实验步骤 Part I. Cell Lysate Preparation
MATERIALS T-cell: CD2 IgG2b, 01171D B-cell: B220 (CD45R) IgG2a, 01121A CD3 IgG2b, 28001D Monocytic: Mac1 (CD11b) IgG2b, 01711A CD5 IgG2a, 01031
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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