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- Reddot Biotech
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- RD-Alk-Smase-Mu-96T
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Mouse Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96T
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
al 1992, Vaysse and Goldman, 1990). The indigo product of X-gal absorbs in the wavelengths emitted by the standard fluorescent-conjugated antibodies and is so dark that it can obscure the products from either horseradish peroxidase or alkaline
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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