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- 文献和实验
- 技术资料
- 英文名:
Bacillus megaterium
- 保质期:
1个月
- 供应商:
智立中特(武汉)生物科技有限公司
- 保存条件:
2-8度
- 规格:
冻干粉
Name:
Bacillus megaterium de Bary 1884
DSM No.:
322
Isolated from:
garden soil
Country:
Germany
G?ttingen
Date of sampling:
before 22.08.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- C. Schaab
Cultivation conditions:
Medium 1 , 30°C
Complete DSMZ Media List
Summary and
additional information:
<- C. Schaab. Garden soil. Taxonomy/description (2413). (Medium 1, 30°C).
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
2413
Risk group:
1 (classification according to German TRBA)
Supplied as:
Delivery form Prices
Price Category for this culture: 1
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文献和实验Name:
Bacillus megaterium de Bary 1884
DSM No.:
322
Isolated from:
garden soil
Country:
Germany
G?ttingen
Date of sampling:
before 22.08.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- C. Schaab
Cultivation conditions:
Medium 1 , 30°C
Complete DSMZ Media List
Summary and
additional information:
<- C. Schaab. Garden soil. Taxonomy/description (2413). (Medium 1, 30°C).
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
2413
Risk group:
1 (classification according to German TRBA)
Supplied as:
Delivery form Prices
Price Category for this culture: 1
以下操作按照天根产品 DP322 口腔拭子基因组 DNA 提取试剂盒的说明书进行,所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品,样本量不同可能现象与本文有所差异。实验准备:1. 口拭子2. 移液器及配套无菌枪头(200 μl ,1ml),2 ml离心管3. 无水乙醇4. 涡旋振荡器,金属浴/水浴,台式离心机注意:为了保证样本不被食物或者饮料污染,取样前 30 min内请勿进食和饮水。实验准备-试剂盒准备:使用前先在漂洗液PW和GD中加入无水乙醇,加入体积请参照瓶
Back to Basics: pBR322 and Protein Expression Systems in E. coli
plasmid-borne elements and their interaction with the particular host strain will influence the overall expression system and final productivity (3 ) (see Chapters 1 –3 ). Plasmid vector pBR322 (4 ) is a well-established multipurpose cloning
1),尿嘧啶合成酶基因(URA3),亮氨酸合成酶基因(LEU2),组氨酸合成酶基因(HIS4)等。 3) 生化标记基因。这类标记基因的表达产物可催化某些易检测的生化反应,常用的基因有β-半乳苷酶基因(lacZ)、葡萄糖苷酸酶基因(GUS)、氯霉素乙酰转移酶基因(CAT)。 为了进一步说明载体的结构和各个结构的功能,下面举一个在基因工程中使用比较频繁的大肠杆菌载体—pBR322作为例子。 大肠杆菌载体pBR322 载体pBR322是由Bolivar 等人于1977年构建的。该载
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