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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
详询
- 英文名：
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50Preps
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文献和实验Isolation of Cell-Free RNA from Maternal Plasma
of cell-free RNA from plasma samples, and we have found the protocol using TRIzol LS reagent (Invitrogen) as lysis buffer combined with RNeasy Mini kit (QIAGEN) to be the optimal method for extracting high-quality cell-free RNA in the highest quantities
containing100µl (6-7×108 beads/ml) of Dynabeads™ M-280 Streptaviden (Figure 1b).Pulldown (3), RNA-biotin specific Incubate the elute/Dynabead™ slurry at 4°C for 15 minutes on an orbital shaker followed by capture with a magnetic bead separator. The captured
and process following the protocol described in the PureLink™ Micro-to-Midi™ RNA Purification System manual available there . Determine the quality and quantity of the purified RNA using UV absorbance at 260 nm or Quant-iT™ RNA Assay Kit (cat
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