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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80
- 保质期：
详询
- 英文名：
【干冰】TruScript First Strand cDNA Synthesis Kit for mRNA
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50Reactions
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文献和实验Protocol for First-strand cDNA Synthesis
Prepare in a sterile tube:template RNA:total RNA 0.1-5µgor poly(A)+ mRNA 10ng-0.5µg,or specific RNA 0.01pg-0.5µgprimer:oligo(dT)18 0.5µgor random hexamer 0.2µg,or sequence-specific 15-20pmol,deionized water (nuclease free) up to 11µl.Incubate
transcriptase and the bead-bound oligo (dT) as primer for first strand cDNA synthesis, an initial incubation at 50°C for 5 minutes is necessary before proceeding at the recommended temperature. 8) If elution of mRNA from the beads is desired, add
of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
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