【干冰】Small RNA Library Prep Kit

Native Chromatin Preparation and Illumina/Solexa Library Construction

DNA Repair and Solexa Library Construction   39. Repair DNA ends to generate blunt-ended DNA using the DNA End-Repair Kit (Epicentre). Combine: 1-34 µL of DNA (0.3 µg) 5 µL of 10X End-Repair

Summary: Illumina's Solexa Sequencing Technology

fragments of the size range of 150-200bp are isolated via gel extraction and amplified using limited cycles of PCR.Complete detailed protocols for DNA and small RNA library preparation can be found in the documents provided in the attachments to this post

Small RNA Library Construction for High-Throughput Sequencing

sequencing provides the requisite level of resolution that other procedures, like northern blotting, would not be able to achieve. Here, we describe a protocol for the preparation of small RNA libraries for sequencing using the Solexa/Illumina technology.