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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
sulfo-Cyanine5 bis-NHS ester, 5 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
5mg
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文献和实验Preparation Of Peptide-KLH Conjugates For Immunization
.(6) For the -SH coupling, warm the other aliquot of KLH to room temp. Add 1/9 th volume of Iodoacetic acid N-hydroxysuccinimide ester at 100mg/ml in DMSO. Make the DMSO stock fresh, and protect the iodoacetamide reagent from light. We make our own IAA-NHS ester
Preparation Of Peptide-KLH Conjugates For Immunization
of KLH to room temp. Add 1/9 th volume of Iodoacetic acid N-hydroxysuccinimide ester at 100mg/ml in DMSO. Make the DMSO stock fresh, and protect the iodoacetamide reagent from light. We make our own IAA-NHS ester, but it can be purchased from Sigma
Coupling Peptides To Resin For Affinity Purification
for 10 min at RT. The resin has about 10 5mol groups/ml, so add about 20 5mol reagent per ml (7 mg/ml resin). Dissolve the IAA-NHS ester in dry DMSO and add it while stirring the resin. This step and subsequent steps up to the blocking of residual
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