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- 文献和实验
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- 保存条件：
详询
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- 英文名：
ROX azide, 5-isomer, 25 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
25mg
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
cells per time point. (Raffinose has not worked well for me, so I avoid it.) 2. Arrest the culture with alpha factor (200 nM final concentration for bar1 strains) when the OD660 is about 0.2-0.25. 3. When the cells have arrested (more than 95
SYBRGreen Q-PCR in the ABI 7700
Materials ROX Passive Reference Dye 25 µM, 50x (Invitrogen, Cat. No. 12223-012 ) SYBR Green I, "10,000x" concentrate (Molecular Probes, Cat. No. S7563 ) SureStart Taq polymerase 5 U/µL (Stratagene, Cat. No. 600280 ) Solutions SYBR
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