FAM azide, 5-isomer, 100 mg

Replication timing by density transfer

. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289

Purification of Antiserum or Ascites by Protein A/G Chromatography

A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG

DETECTION OF RECOMBINANT PROTEINS BY ELISA

2CO 3 (0.012M) 0.02 g MgCl 2 (100 µl 1 M stock; 1 mM) 200 µl 10% sodium azide Add Na 2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml