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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
FAM azide, 5-isomer, 10 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
10mg
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES: 50 mM Tris Cl pH 9.5 5 mM MgCl 2% agarose -dissolve 2 mg n-propyl gallate in 70
Replication timing by density transfer
(frozen slanted to maximize the surface area). During the experiment, mix 20 ml of cells with 1/50 volume of 10% Na-azide in a 35 ml Corex tube (on ice) and immediately transfer the mix to a tube of frozen EDTA/azide. Alternatively, squirt the 10% azide
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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