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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
FAM azide, 5-isomer, 100 uL, 10 mM/DMSO
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
5-isomer
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文献和实验FAM caspase 8 and 9 binding assay for embryos
is available. MATERIALS Preparation of 150X FAM Reagent Frozen Aliquot Preparation (Store frozen) Add 50 µl of DMSO to one vial of lyophilized reagent. Mix until dissolved. Aliquot 10 µl into tubes for later use. Freeze
oligonucleotides. Melting curves were measured on Cary 400 Scan UV‐Visible Spectrophotometer (Varian) at 5 to 7 µM oligonucleotide in 10 mM phosphate buffer/200 mM NaCl, pH 7.0, to which increasing amounts of formamide were added. Spectra were recorded at 272 nm. T
Staining Methods for cell death Z. Xia 10/2/95
gently tilt the plates to mix R.T. 15 min Aspirate off media Staining: 4 ug/ul P. I./0.1 % triton X-100/0.5 mg/ml RNaseA in PBS R.T.5 min Examine under the scope or mount with coverslips Note: P
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