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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
FAM-11-UTP, 6-isomer, 250 nmol
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
250nmol
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文献和实验). (a ) Commercial UTP (retention time: 14.1 min); (b ) crude synthesized UTPαS following NaCl/ethanol precipitation, showing diastereomers (retention times: 17.5 and 18.3 min); (c ) HPLC‐purified diastereomer of UTPαS (peak I, S p isomer, retention time: 15.6 min
The ribonuclease protection assay (RPA)
, the probe synthesis is performed during the afternoon Day 1, hybridizations are incubated overnight, and RNase treatments and gel electrophoresis are performed early on Day 2. Probe Synthesis: 1. Bring the [a-32P]UTP, GACU nucleotide pool, DTT
dATP 1 mM dCTP 1 mM dGTP 0.65 mM dTTP 0.35 mM alkali-labile digoxigenin (dig)-UTP (B-Mann cat# 1573152 or 1573179) *supplied in the dig DNA labelling kit; also can order all tubes separately, i.e. without enzyme. 2. Reaction: a. Heat at 100 deg C
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