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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
FAM-11-dUTP, 6-isomer, 250 nmol
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
250nmol
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文献和实验) by digoxigenin-11-dUTP (DIG-dUTP) for use as a labelling mix for PCR labelling of PCR products:GIVEN: DIG-dUTP supplied (by Boehringer Mannheim) at 25 nmol/25ul = 1 umol/ml = 1mM; final concentration of DIG-dUTP must be 1/10th that of other nucleotides, and [DIG
. expressed in EACH dNTP). THEN dilute this (or aliquot) 1/10 with WATER - aliquot into 100 ul amounts and freeze. ii) Prepare a 1 mM stock of dNTPs with dTTP substituted to 10% (w/w) by digoxigenin-11-dUTP (DIG-dUTP) for use as a labelling mix for PCR
Calculating Concentrations for PCR
this (or aliquot) 1/10 with WATER - aliquot into 100 ul amounts and freeze. ii) Prepare a 1 mM stock of dNTPs with dTTP substituted to 10% (w/w) by digoxigenin-11-dUTP (DIG-dUTP) for use as a labelling mix for PCR labelling of PCR products: GIVEN: * DIG-dUTP
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