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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
AF 555 NHS ester, 100 mg
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 4.3 mM CaCl2,1.2 M KI,50 mM Tris-HCl,pH 8.0,5 ml. 5.0.5 mM ATP,0.2 mM CaCl2,0.5 mM DTT,2 mM Tris-HCl,pH 8.0 at 4℃,1 liter. 6.CFSE (Molecular Probes,C-1311; not the diacetate),prepare a stock solution of 50 mg/ml in DMSO. 7.Small SS34 tubes and adaptors
Labeling Tubulin and Quantifying Labeling Stoichiometry
and stoichiometry for tubulin labeled with tetramethylrhodamine (TMR) NHS ester: Tubulin concentration = [(A280 - Contribution of dye to A280 ) x Dilution Factor] / Extinction coefficient of tubulin at 280 nm TMR concentration = (A555 x Dilution Factor
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