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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Lactoferricin, Bovine, mAb 5F12.1.2, 20 ug
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
20UG
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文献和实验ddH2O and filter as before. pH=3.0.(11) Regeneration buffer (BioRad): BioRad Affi-gel regeneration buffer.2. Procedure(1) For Ammonium sulfate cutFilter Sup. in 1L Costar Filter using pre-filter.Add 50 mM Tris pH7.8 (50 ml of 1 M stock/Liter): 100
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
Reverse Transcription of RNA- 20l rxn
for 10mins. Notes All reagents should be diluted in dH20 that has been dep''d and autoclaved. Add all reagents except the RT and RNA and let them sit in the presence of the RNase for 15 mins. I use 1.5 ug of Poly A+ RNA in this rxn
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