CD55, Rat, mAb RDII-24, 20 ug

24h Subcloning Protocol

of (I) and (V) to obtain compatible overhangs (even blunt ligations work well with this protocol).  Generally, perform restriction digests as follows:           DNA                               1-10 ul (2 ug TOTAL)    

24cm IPG胶条二维电泳

实验步骤   1. 清洗器具用品：清洗所有的干胶条套件，包括胶条槽、盖片、托盘等。用Ettan IPGphor胶条槽清洗剂(Amersham Biosciences.)，并用大量去离子水冲洗，自然干燥备用。 2. 第一向等电聚焦电泳    1) 加样和IPG干胶条再水化：采用样品加在再水化液中方法进行加样。24cm IPG胶条(PH3 -10Non-line )。银染蛋白质上样量为40ug-50ug。根据上样量计算出样品

Transfection of Primary Cultures of Rat Hepatocytes

of male rat hepatocytes. Hepatocytes were isolated by the collagenase perfusion method and then cultured on Matrigel-coated plates for 24 h before transfection. The cells were transfected with either pGL3-Control or pGL3-Basic plasmids. The efficiency