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- 保存条件:
详询
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- 英文名:
Mix-n-Stain™ CF®790 Antibody Labeling Kit, 1x(50-100ug) labeling
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
1KIT
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文献和实验and concentrations will remain consistent for the labeling. B. Add Total volume (50µl,) of Tube 1 (TdT) + remainder of Tube 2 (450µl). Apply 100µl TUNEL reaction mixture (or 100µl Control Label solution for negative control) to each slide.
use the BioPrime labeling kit (Gibco/BRL) as a convenient and inexpensive source of random octamers, reaction buffer, and high concentration klenow (do not use the dNTP mix provided in the kit), though other sources of random primers and high
Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
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