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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
gPIG-ZAP Antibody Internalization Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1kit,tests1ab
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文献和实验Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells
in internalization of the antibody. This allows use of the antibody to deliver toxic payloads intracellularly to achieve a therapeutic effect. Here we describe how phage antibody libraries can be directly selected on tumor cell lines to generate antibodies binding
effects on tumor cells. This chapter describes some of the techniques that are currently used to determine internalization and proper intracellular trafficking of antibodies in order to select an optimal antibody for ADC therapeutics.
Flow Cytometry Assay to Measure Internalization of GPCRs
Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cytometer. The antibodies recognize an epitope
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