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- Abbexa
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- abx710063-100ml
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store the basal medium at 4 °C, and all other solutions at -20 °C. Avoid exposure to light.
- 保质期：
详询
- 英文名：
【干冰】Medium for Mouse Pulmonary Fibroblasts (PF)
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100ml
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文献和实验Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
You will need: 13.5 day pregnant mouse (we use MTK NEO inbred white mice) 2 sets sterile instruments one containing a pair of curved forceps and a pair of iris scissors one containing two pairs curved forceps, one pair iris
Isolation of Primary Fibroblasts from Mouse Embryos
among three 10 cm gelled plates, each containing 8 ml of medium (DMEM, 1X GPS, and 10% FCS). These plates are designated as Passage No. 1. 10. Upon confluence, harvest and freeze the primary fibroblasts @ a freezing density of 3.0 x 107 cells/ml. Reserve
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