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T25
mpc-5/mpc-5细胞系/mpc-5细胞株/mpc-5小鼠肾小球足细胞
Cell line name MPC-5
Synonyms MPC5; Mouse Podocyte Clone-5
Accession CVCL_AS87
Resource Identification Initiative To cite this cell line use: MPC-5 (RRID:CVCL_AS87)
Comments Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Podocyte; CL=CL_0000653.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (CBA/Ca x C57BL/10)Tg(H2Kb-tsA58) Immortomouse.
Hierarchy Children:
CVCL_E0ZA (Ubigene MPC-5 Tollip KO)
Category Conditionally immortalized cell line
STR profile Source(s): Ubigene=YC-A104
Markers:
Mouse STR 4-2 19.3,20.3
Mouse STR 5-5 14
Mouse STR 6-4 18
Mouse STR 6-7 12,17
Mouse STR 12-1 17
Mouse STR 15-3 22.3,26.3
Mouse STR 18-3 16
Mouse STR X-1 26,28
Run an STR similarity search on this cell line
Publications
PubMed=9344605; DOI=10.1006/excr.1997.3739
Mundel P., Reiser J., Zuniga Mejia Borja A., Pavenstadt H., Davidson G.R., Kriz W., Zeller R.
Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines.
Exp. Cell Res. 236:248-258(1997)
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文献和实验*发表【中文论文】请标注:由博辉生物科技(广州)有限公司提供; *发表【英文论文】请标注:From Bohui Biological Technology (Guangzhou) Co., Ltd.
PNAS:RAS 抑制剂又添一员,新型蛋白模拟物或为癌症治疗
12X 突变体 H-Ras 与 CHDSos-5 的肽滴定实验表明,蛋白模拟物在类似的低微摩尔亲和力范围内结合所有 Ras 蛋白。随后研究团队评估了 Sos 蛋白模拟物抑制细胞中 Ras 信号转导的潜力。MTT 细胞活性实验发现,与野生型细胞系相比,CHDSos-5 对含有致癌 Ras 突变的细胞系表现出浓度依赖性毒性,并且细胞活性与不同细胞系间固有的巨胞饮摄取水平呈负相关。这些结果表明,利用上调的巨胞饮作用为突变癌细胞提供治疗提供了潜在优势。图片来源:PNAS化学蛋白质组学分析揭示了 CHDSos-5
磁珠,并可接受订制服务。三、Dynabeads的使用方法Dynabeads可加入到不同种类的悬浮液中以结合靶物质(如细胞、核酸、细菌或其他生物分子),形成一种Dynabeads·靶物质混合物,在通过磁分离器(如Dynal MPC)后可以将该混合物从悬液中移去。四、Dynal MPC 磁分离器的优点1、操作简便2、磁性强久3、适合多种体积(50μl~50ml)4、分离迅速目录号产品说明规格价格(RMB)120.01Dynal MPC-1 for 5ml - 50ml tubes1 unit
RLCS (Restriction Landmark cDNA Scanning) Protocol
or mixer for 3-5 min at room temperature. 12. Place tube in MPC-M for 2 min and remove supernatant. Transfer the tube to another rack. 13. Wash twice with 1 ml Washing buffer with LiDS at room temperature using MPC-M and once 1mL Washing buffer.
技术资料






