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T25
IPEC-J2细胞IPEC-J2细胞IPEC-J2；猪小肠上皮细胞
Cell line name IPEC-J2
Synonyms IPECJ2; Intestinal Porcine Epithelial Cell line-J2
Accession CVCL_2246
Resource Identification Initiative To cite this cell line use: IPEC-J2 (RRID:CVCL_2246)
Comments Characteristics: Undergoes a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high transepithelial electrical resistance (TEER), depending on the type of serum added to the culture medium, within 1-2 weeks (PubMed=29787056).
Virology: Susceptible to infection by porcine circovirus type 2 (PCV2) (PubMed=25407967).
Doubling time: ~40-60 hours (DSMZ=ACC-701).
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Small intestine, jejunum; UBERON=UBERON_0002115.
Cell type: Enterocyte; CL=CL_0000584.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Hierarchy Children:
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Publications
DOI=10.1016/S0016-5085(89)80002-2
Berschneider H.M.
Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl.
Gastroenterology 96:A41-A41(1989)
PubMed=16215741; DOI=10.1007/s00418-005-0067-z
Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S., Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K., Wieler L.H.
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Histochem. Cell Biol. 125:293-305(2006)
PubMed=21626283; DOI=10.1007/s10616-011-9362-9; PMCID=PMC3140839
Geens M.M., Niewold T.A.
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Cytotechnology 63:415-423(2011)
PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017; PMCID=PMC3289732
Brosnahan A.J., Brown D.R.
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Vet. Microbiol. 156:229-237(2012)
PubMed=22193509; DOI=10.1007/s10616-011-9420-3; PMCID=PMC3397115
Steube K.G., Koelz A.-L., Uphoff C.C., Drexler H.G., Kluess J., Steinberg P.
The necessity of identity assessment of animal intestinal cell lines: a case report.
Cytotechnology 64:373-378(2012)
DOI=10.1155/2013/980651
Stoy A.C.F., Heegaard P.M.H., Sangild P.T., Ostergaard M.V., Skovgaard K.
Gene expression analysis of the IPEC-J2 cell line: a simple model for the inflammation-sensitive preterm intestine.
ISRN Genomics 2013:980651.1-980651.7(2013)
PubMed=24260272; DOI=10.1371/journal.pone.0079643; PMCID=PMC3829867
Zakrzewski S.S., Richter J.F., Krug S.M., Jebautzke B., Lee I.-F.M., Rieger J., Sachtleben M., Bondzio A., Schulzke J.-D., Fromm M., Gunzel D.
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
PLoS ONE 8:E79643-E79643(2013)
PubMed=25407967; DOI=10.1186/s12985-014-0193-0; PMCID=PMC4237784
Yan M.-F., Zhu L.-Q., Yang Q.
Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments.
Virol. J. 11:193.1-193.9(2014)
PubMed=26147118; DOI=10.1371/journal.pone.0132323; PMCID=PMC4493080
Nossol C., Barta-Boszormenyi A., Kahlert S., Zuschratter W., Faber-Zuschratter H., Reinhardt N., Ponsuksili S., Wimmers K., Diesing A.-K., Rothkotter H.-J.
Comparing two intestinal porcine epithelial cell lines (IPECs): morphological differentiation, function and metabolism.
PLoS ONE 10:E0132323-E0132323(2015)
PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12
Vergauwen H.
The IPEC-J2 cell line.
(In book chapter) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.125-134; Springer; Cham; Switzerland (2015)
PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874
Saaby L., Helms H.C.C., Brodin B.
IPEC-J2 MDR1, a novel high-resistance cell line with functional expression of human P-glycoprotein (ABCB1) for drug screening studies.
Mol. Pharm. 13:640-652(2016)
PubMed=26975772; DOI=10.4014/jmb.1512.12075
Kim S.H., Pajarillo E.A.B., Balolong M.P., Lee J.Y., Kang D.-K.
Constructing proteome reference map of the porcine jejunal cell line (IPEC-J2) by label-free mass spectrometry.
J. Microbiol. Biotechnol. 26:1124-1131(2016)
PubMed=32708885; DOI=10.3390/pharmaceutics12070673; PMCID=PMC7408396
Saaby L., Trasborg J., Rasmussen M.A., Holst B., Brodin B.
IPEC-J2 rMdr1a, a new cell line with functional expression of rat P-glycoprotein encoded by rat Mdr1a for drug screening purposes.
Pharmaceutics 12:673.1-673.17(2020)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
Cell：「来骗来偷袭」剪接体靶向疗法「诱骗」机体，激活抗病毒免疫，让肿瘤自杀
传导以往研究表明，MYC 诱导的三阴性乳腺癌（triple-negative breast cancer，TNBC）对剪接体的基因扰动（genetic perturbation）很敏感，然而对剪接体抑制产生应答的途径仍然未知。基于此，作者首先对两个 MYC 诱导的 TNBC 细胞系 SUM159 和 LM2 的转录变化进行了表征，然后用小分子剪接体调节剂 SD6 进行处理。基因富集分析发现免疫信号通路（干扰素 α 和 β 信号通路）是正向富集最显著的途径，另外干扰素刺激基因 (如 OAS1、MX1
领域奠基之作：Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
唾液酸形式 Neu5Ac 和 Neu5Gc。而当样品被 VC-Sia 或 RNase 处理时，则检测不到，进一步证实 glycoRNA 被唾液酸修饰的。 图片来源：Cell 经典的 N - 多糖合成机制有助于 glycoRNA 的产生 蛋白质上有两类主要的多糖，N - 多糖和 O - 多糖，两者都可以被唾液酸化。 研究人员使用遗传学、药理学和酶学的结合方法进行检测，探索 glycoRNA 结构是否与糖蛋白相关多糖结构相关。 IdID CHO 细胞系不能产生 UDP-Gal 和 UDP
www.33ge.com `7j5c Nt8l -o 肿瘤细胞培养 P.} 分析化学, 论坛, 化学分析, 仪器分析, 分析测试, 色谱, 电泳, 光谱#t$d%m"?4d 肿瘤细胞在组织培养中占有核心的位置，首先癌细胞是比较容易培养的细胞。当前建立的细胞系中癌细胞系是最多的。另外肿瘤对人类是威胁最大的疾病。肿瘤细胞培养是研究癌变机理、抗癌药检测、癌分子生物学极其重要的手段。肿瘤细胞培养对阐明和解决癌症将起着不可估量的作用。 | 分析
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