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T25
MDBK细胞MDBK细胞MDBK;牛肾细胞
Cell line name MDBK
Synonyms MDBK (NBL-1); NBL-1; Madin-Darby Bovine Kidney; Madin Darby Bovine Kidney
Accession CVCL_0421
Resource Identification Initiative To cite this cell line use: MDBK (RRID:CVCL_0421)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: Not susceptible to infection by wild-type and vaccinal strains of myxoma virus (MYXV) (PubMed=17900332).
Doubling time: ~35 hours (DSMZ=ACC-174); ~24 hours (Note=Lot 032699), ~31 hours (Note=Lot 07222019) (JCRB=JCRB9028).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Hierarchy Children:
CVCL_DD22 (BHH2C) CVCL_DD23 (BHH3) CVCL_DD24 (BHH8)
CVCL_A9KJ (BTL2013) CVCL_DD19 (CRIB-1) CVCL_DD25 (M2B)
CVCL_E1FE (MDBK CD46 KO) CVCL_DD20 (MDBK clone 955-1) CVCL_DD21 (MDBK clone 958-9)
CVCL_VD89 (MDBK DeltaGPI) CVCL_RT35 (MDBK-221) CVCL_4541 (MDBK-Bu100)
CVCL_A2ZW (MDBK-N4) CVCL_A9LD (MDBK-Nectin-4) CVCL_E073 (MDBK-NST)
CVCL_0I74 (MDBK-PES) CVCL_RX00 (MDBK-t2) CVCL_A2ZX (MDBK-TfR)
CVCL_3021 (MDBK.P3) CVCL_IZ74 (MDBK/441) CVCL_B7IL (MDBK527)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications
PubMed=13567776; DOI=10.3181/00379727-98-24111
Madin S.H., Darby N.B. Jr.
Established kidney cell lines of normal adult bovine and ovine origin.
Proc. Soc. Exp. Biol. Med. 98:574-576(1958)
PubMed=14207851; DOI=10.1093/jnci/33.2.347
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Preservation of bulk chromatin with decrease in number of chromosomes in cells of an established bovine kidney line.
J. Natl. Cancer Inst. 33:347-361(1964)
PubMed=5988125; DOI=10.1007/BF00326444
Nelson-Rees W.A., Darby N.B. Jr., Siegel S.T.
Clonal derivatives of a bovine kidney cell line (MDBK) and continuing preservation of total chromosomal material.
Chromosoma 18:70-78(1966)
PubMed=5587551; DOI=10.1159/000129961
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Debut and accumulation of centric fusion products: an index to age of certain cell lines.
Cytogenetics 6:436-450(1967)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=2580922; DOI=10.1089/jir.1985.5.65
Feinstein S., Traub A., Lazar A., Mizrahi A., Teitz Y.
Studies on cell binding and internalization of human lymphoblastoid (Namalva) interferon.
J. Interferon Res. 5:65-76(1985)
PubMed=17900332; DOI=10.1186/1743-422X-4-94; PMCID=PMC2045666
Pignolet B., Duteyrat J.-L., Allemandou A., Gelfi J., Foucras G., Bertagnoli S.
In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains.
Virol. J. 4:94.1-94.5(2007)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=24973239; DOI=10.1099/vir.0.065995-0
Richter M., Reimann I., Schirrmeier H., Kirkland P.D., Beer M.
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)
PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)
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文献和实验*发表【中文论文】请标注:由博辉生物科技(广州)有限公司提供; *发表【英文论文】请标注:From Bohui Biological Technology (Guangzhou) Co., Ltd.
edwardellen 求助!!! 本人菜鸟,最近开始接触凋亡相关信号,在HepG-2中做实验,有一点很困惑,HepG-2中的p53是野生型的还是突变型的?还是两者都存在?查了一些资料,有些文章之间表达的意思模凌两可。困惑啊! 哪位好心的达人能给我一个确定的答案呢? 另外,是否有做p53相关的牛人能告诉我P53在哪些实验用肿瘤细胞系中是野生型的,在哪些是突变型的? 谢谢!谢谢!谢谢! doctormy
shao74 最近在做细胞凋亡方面的实验,碰到的问题是手头上的细胞系对凋亡不是很敏感,所以不知哪位有更好的建议,谢谢! freecell 这个不好说啊,看你主要做哪个组织的细胞凋亡,什么因素引起的细胞凋亡。 Fasta921 所以要自己筛选敏感的细胞株啊,不同细胞系对同种基因或药物的耐受性不一样的。 goldendoctor Hela 是比较常用
各种已被命名和经过细胞生物学鉴定的细胞系或细胞株 ,都是一些形态比较均一、生长增殖比较稳定的和生物 性状清楚的细胞群。因此凡符合上述情况的细胞群也可 给以相应的名称,即文献中常称之为已鉴定的细胞(Certified Cells)。已鉴定的细胞可用于各种实验研究和生产生物制品。当前世界上已建的各种细胞系(株)已难胜数,我国也建有百种以上,并在不断增长中。体外培养细胞的种类和命名 体外培养细胞的名称,随培养细胞技术的发展和细胞种类的增多而演变。最早采用的名称为细胞株(Cell strain),以后
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