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T25
UMR-106/UMR-106细胞系/UMR-106细胞株/UMR-106大鼠骨肉瘤细胞
Cell line name UMR-106
Synonyms UMR 106; UMR106
Accession CVCL_3617
Resource Identification Initiative To cite this cell line use: UMR-106 (RRID:CVCL_3617)
Comments Characteristics: Established from a transplantable tumor.
Characteristics: Responsive to PTH, prostaglandins and bone-resorbing steroids.
Transformant: NCIt; C176700; Phosphorus-32 beta radiation.
Derived from site: In situ; Bone; UBERON=UBERON_0002481.
Disease Rat osteosarcoma (NCIt: C60487)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS:0000681.
Hierarchy Children:
CVCL_E1EU (Ubigene UMR-106 CVCL_D580 (UMR-106-01) CVCL_S477 (UMR-106-06)
Originate from same individual CVCL_S478 ! UMR-104
CVCL_S479 ! UMR-105
CVCL_3618 ! UMR-108
Age at sampling Adult
Category Cancer cell line
Publications
PubMed=6575864
Partridge N.C., Alcorn D., Michelangeli V.P., Ryan G., Martin T.J.
Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin.
Cancer Res. 43:4308-4314(1983)
PubMed=7988420; DOI=10.1210/endo.135.6.7988420
Williams G.R., Bland R., Sheppard M.C.
Characterization of thyroid hormone (T3) receptors in three osteosarcoma cell lines of distinct osteoblast phenotype: interactions among T3, vitamin D3, and retinoid signaling.
Endocrinology 135:2375-2385(1994)
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文献和实验*发表【中文论文】请标注:由博辉生物科技(广州)有限公司提供; *发表【英文论文】请标注:From Bohui Biological Technology (Guangzhou) Co., Ltd.
Neuroendocrine regulation of cyclic AMP formation in osteoblastic cell lines (UMR-106-01, ROS 17/2.8, MC3T3-E1, and Saos-2) and primary bone cells.The effect of four different neuropeptides and norepinephrine (NE) on cyclic AMP formation
Emmanuel SURANITI1, Elodie SOLLIER2, Roberto CALEMCZUK1, Thierry LIVACHE1, Patrice MARCHE2, Marie-Bernadette VILLIERS2, Yoann ROUPIOZ1 1 CREAB,UMR 5819 (CEA-CNRS-UJF), DRFMC, CEA Grenoble France. 2 INSERM U823,equipe 8, Institut Albert
1D4系统的最大优势在于抗体与抗原相互作用的高度特异性。表位序列和链长对结合至关重要。例如,将第三位丙氨酸替换成甘氨酸,即移去一个甲基基团,抗体将不再与表位结合。同样,完整的九个氨基酸标签会与Rho1D4抗体结合紧密,去除两个氨基酸则阻断结合。因此,含有与Rho1D4表位相似序列的蛋白引起的非特异性结合被最小化,因此回收蛋白的纯度非常高。(见表1) 3,4,5,6,7,8高回收量而Rho1D4系统的另一个优势是洗脱目标蛋白的高回收率。包括细菌,酵母和哺乳动物细胞系在内的表达系统已经针对特定的GPCR
技术资料





