MOLM13细胞

MOLM13/MOLM13细胞系/MOLM13细胞株/MOLM13急性髓细胞样白血病

Cell line name MOLM-13

Synonyms MOLM13; Molm13; Molm 13

Accession CVCL_2119

Resource Identification Initiative To cite this cell line use: MOLM-13 (RRID:CVCL_2119)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: LL-100 blood cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Population: Japanese.

Doubling time: 3-4 days (PubMed=9305600); 24 hours (PubMed=25984343); 41.1 hours (PubMed=28052028); ~50 hours (DSMZ=ACC-554).

Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526; Sanger).

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.

Sequence variations

Gene fusion; HGNC; 7132; KMT2A + HGNC; 7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (PubMed=9305600; PubMed=14671638; PubMed=15381384).

Mutation; HGNC; 3765; FLT3; Unexplicit; Internal tandem duplication (FLT3-ITD); ClinVar=VCV000016270; Zygosity=Unspecified (PubMed=10233379; PubMed=12529668; PubMed=27750403).

HLA typing Source: DSMZCellDive=ACC-554

Class I

HLA-A A*26:01:01,31:01:02

HLA-B B*44:03:01,51:01:01

HLA-C C*14:02:01,14:03

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0

Native American 1.24

East Asian, North 76.64

East Asian, South 22.12

South Asian 0

European, North 0

European, South 0

Disease Adult acute myeloid leukemia (NCIt: C9154)

Myelodysplastic syndrome (NCIt: C3247)

Acute myeloid leukemia (ORDO: Orphanet_519)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_D779 (MOLM-13-RES) CVCL_C0WJ (MOLM-13/AZA) CVCL_C0WK (MOLM-13/DAC)

CVCL_WI20 (MOLM/AZA-1) CVCL_WI21 (MOLM/DEC-5) CVCL_RS50 (MOLM13rARA-C2mug/ML)

Originate from same individual CVCL_7916 ! MOLM-14

Sex of cell Male

Age at sampling 20Y

Category Cancer cell line

STR profile Source(s): AddexBio=C0003003/60; COG; Cosmic-CLP=1330947; DSMZ=ACC-554; JCRB=JCRB1810; PubMed=25877200

 

Markers:

Amelogenin X,Y

CSF1PO 10 (AddexBio=C0003003/60)

10,12 (COG; Cosmic-CLP=1330947; DSMZ=ACC-554; JCRB=JCRB1810; PubMed=25877200)

D2S1338 23,25

D3S1358 15

D5S818 10,11

D7S820 10,12

D8S1179 13,14

D13S317 10,11

D16S539 10,11

D18S51 13,15

D19S433 12,14

D21S11 30,31

FGA 21,23

Penta D 9,12

Penta E 18,19

TH01 7

TPOX 8

vWA 16,17

 

Run an STR similarity search on this cell line

Publications

PubMed=9305600; DOI=10.1038/sj.leu.2400768

Matsuo Y., MacLeod R.A.F., Uphoff C.C., Drexler H.G., Nishizaki C., Katayama Y., Kimura G., Fujii N., Omoto E., Harada M., Orita K.

Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23).

Leukemia 11:1469-1477(1997)

 

PubMed=10233379; DOI=10.1111/j.1365-2141.1999.01284.x

Xu F., Taki T., Yang H.W., Hanada R., Hongo T., Ohnishi H., Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.

Tandem duplication of the FLT3 gene is found in acute lymphoblastic leukaemia as well as acute myeloid leukaemia but not in myelodysplastic syndrome or juvenile chronic myelogenous leukaemia in children.

Br. J. Haematol. 105:155-162(1999)

 

PubMed=10637496; DOI=10.1038/sj.leu.2401604

Drexler H.G., Fombonne S., Matsuo Y., Hu Z.-B., Hamaguchi H., Uphoff C.C.

p53 alterations in human leukemia-lymphoma cell lines: in vitro artifact or prerequisite for cell immortalization?

Leukemia 14:198-206(2000)

 

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.

Leuk. Res. 24:255-262(2000)

 

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

 

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.

Leuk. Res. 25:275-278(2001)

 

PubMed=12529668; DOI=10.1038/sj.leu.2402740

Quentmeier H., Reinhardt J., Zaborski M., Drexler H.G.

FLT3 mutations in acute myeloid leukemia cell lines.

Leukemia 17:120-124(2003)

 

PubMed=14504097; DOI=10.1182/blood-2003-02-0418

Taketani T., Taki T., Sugita K., Furuichi Y., Ishii E., Hanada R., Tsuchida M., Sugita K., Ida K., Hayashi Y.

FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.

Blood 103:1085-1088(2004)

 

PubMed=14671638; DOI=10.1038/sj.leu.2403236

Drexler H.G., Quentmeier H., MacLeod R.A.F.

Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.

Leukemia 18:227-232(2004)

 

PubMed=15381384; DOI=10.1016/j.cancergencyto.2004.01.029

Montemurro L., Tonelli R., Fazzina R., Martino V., Marino F., Pession A.

Identification of two MLL-MLLT3 (alias MLL-AF9) chimeric transcripts in the MOLM-13 cell line.

Cancer Genet. Cytogenet. 154:96-97(2004)

 

PubMed=16408098; DOI=10.1038/sj.leu.2404081

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Leukemia 20:471-476(2006)

 

PubMed=22354205; DOI=10.1038/leu.2012.52; PMCID=PMC3523391

Moore A.S., Faisal A., Gonzalez de Castro D., Bavetsias V., Sun C.-B., Atrash B., Valenti M., De Haven Brandon A.K., Avery S., Mair D., Mirabella F., Swansbury J., Pearson A.D.J., Workman P., Blagg J., Raynaud F.I., Eccles S.A., Linardopoulos S.

Selective FLT3 inhibition of FLT3-ITD+ acute myeloid leukaemia resulting in secondary D835Y mutation: a model for emerging clinical resistance patterns.

Leukemia 26:1462-1470(2012)