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T25
【ATCC来源】BALB/3T3 clone A31、BALB/3T3 clone A31、BALB/3T3 clone A31细胞、BALB/3T3 clone A31细胞、BALB/3T3 clone A31BALB/3T3 clone A31[小鼠胚胎成纤维细胞]
Cell line name BALB/3T3 clone A31
Synonyms BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N
Accession CVCL_0184
Resource Identification Initiative To cite this cell line use: BALB/3T3 clone A31 (RRID:CVCL_0184)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: 30-32 hours (Note=At 33 Celsius) (PubMed=4798834); 24 hours (Note=At 38 Celsius) (PubMed=182373).
Omics: SNP array analysis.
Misspelling: BABL/3T3; KCB=KCB 200713YJ.
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Children:
CVCL_6760 (12-A-3) CVCL_VT75 (17-Cl1) CVCL_A6ZR (3T3/40L)
CVCL_VI61 (A31-714) CVCL_A9DY (A31-IS11 EGF-2) CVCL_LF96 (B77-A31)
CVCL_B5VL (BALB/3T3 clone A31 H) CVCL_B5VH (BALB/3T3 clone A31 NTG2) CVCL_B5VD (BALB/3T3 clone A31 Ts-1)
CVCL_B5VE (BALB/3T3 clone A31 ts2) CVCL_B385 (BALB/3T3 clone A31 ts20) CVCL_B5VG (BALB/3T3 clone A31 ts22)
CVCL_A5PN (BALB/3T3 clone A31T) CVCL_4350 (K-BALB) CVCL_5793 (M-MSV-BALB/3T3)
CVCL_LF97 (MC5-5) CVCL_U900 (MT-5) CVCL_T375 (RGB3T3-1)
CVCL_T376 (RGB3T3-5) CVCL_4554 (SV-T2) CVCL_6462 (UC1B)
Sex of cell Sex unspecified
Age at sampling 14-17FD
Category Spontaneously immortalized cell line
STR profile Source(s): PubMed=31220119
Markers:
Mouse STR 1-1 16
Mouse STR 1-2 17
Mouse STR 2-1 16
Mouse STR 3-2 14
Mouse STR 4-2 21.3
Mouse STR 5-5 14
Mouse STR 6-4 18
Mouse STR 6-7 12
Mouse STR 7-1 25.2
Mouse STR 8-1 13
Mouse STR 11-2 17
Mouse STR 12-1 16
Mouse STR 13-1 16.2
Mouse STR 15-3 22.3
Mouse STR 17-2 15,16
Mouse STR 18-3 18
Mouse STR 19-2 14
Mouse STR X-1 25
Run an STR similarity search on this cell line
Publications
PubMed=4301006; DOI=10.1002/jcp.1040720208
Aaronson S.A., Todaro G.J.
Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
J. Cell. Physiol. 72:141-148(1968)
PubMed=4359949; DOI=10.1128/JVI.12.4.711-720.1973; PMCID=PMC356689
Benveniste R.E., Todaro G.J., Scolnick E.M., Parks W.P.
Partial transcription of murine type C viral genomes in BALB c cell lines.
J. Virol. 12:711-720(1973)
PubMed=4792350; DOI=10.1002/ijc.2910120217
Kakunaga T.
A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.
Int. J. Cancer 12:463-473(1973)
PubMed=4798834; DOI=10.1016/0014-4827(73)90283-8
Wittes R.E., Ozer H.L.
Characterization of a temperature-sensitive mutant of BALB/c 3T3 cells.
Exp. Cell Res. 80:127-136(1973)
PubMed=50878
Quarles J.M., Tennant R.W.
Effects of nitrosocarbaryl on BALB/3T3 cells.
Cancer Res. 35:2637-2643(1975)
PubMed=182373; DOI=10.1016/0092-8674(76)90028-3
Slater M.L., Ozer H.L.
Temperature-sensitive mutants of Balb/3T3 cells: description of a mutant affected in cellular and polyoma virus DNA synthesis.
Cell 7:289-295(1976)
PubMed=10848989; DOI=10.1046/j.1432-1327.2000.01404.x
Salvat C., Acquaviva C., Scheffner M., Robbins I., Piechaczyk M., Jariel-Encontre I.
Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo.
Eur. J. Biochem. 267:3712-3722(2000)
PubMed=22576795; DOI=10.3892/or.2012.1763
Ashinuma H., Takiguchi Y., Kitazono S., Kitazono-Saitoh M., Kitamura A., Chiba T., Tada Y., Kurosu K., Sakaida E., Sekine I., Tanabe N., Iwama A., Yokosuka O., Tatsumi K.
Antiproliferative action of metformin in human lung cancer cell lines.
Oncol. Rep. 28:8-14(2012)
PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
该词来源于希腊文的 Kλωγ，原义为小枝之集聚，但后来则指由无性繁殖所产生的同基因型的生物群，这是于 1903年由 H． J． Webber最早应用于生物学上的。过去曾译作具有繁殖意思的营养系或含有原语源意思的分支系（群），而现在则多按其原音直接称为克隆。现在该词在个体、细胞和基因等三方面都使用，而且任何一方面似乎都意味着来自同源的犹如复制一样的同一生物群体。（ 1）关于个体：由无性繁殖所增殖的种群均是克隆，这在植物方面，通过体细胞培养，在试管内繁殖、分化可以重新生出完整
在伯内特（F． M． Burnet）关于免疫的无性繁殖系选择学说中，对自身构成物质有发生免疫反应可能的某淋巴系细胞群，称为禁忌细胞株。禁忌细胞株虽依靠对自身的免疫耐受性而制止活动；但可以假定那种抑制机理一失败，那些细胞就对自身发生免疫反应，而出现自身免疫病。
近日，Cruiser™与市面上易出现假阳性的X错配酶，进行比较，实验结果见下： 下图为3T3细胞FADD基因敲除，已知在3T3细胞中FADD基因的敲除效率很低（其敲除pool测序无双峰），挑24个单克隆后经X酶切结果显示如图2，而经Cruiser™酶切结果显示如图3 图2 3T3细胞FADD基因X酶检测电泳图 图3 3T3细胞FADD基因Cruiser™检测电泳图 P1—Cruiser™酶切阳性对照 P2—X酶切阳性对照 结论：如上图结果显示，X酶
技术资料
