HT22细胞

HT22/HT22细胞系/HT22细胞株/HT22小鼠海马神经元细胞系

Cell line name HT22

Synonyms HT-22

Accession CVCL_0321

Resource Identification Initiative To cite this cell line use: HT22 (RRID:CVCL_0321)

Comments Characteristics: Highly sensitive to glutamate and is therefore used to study glutamate-induced toxicity in neuronal cells.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).

Omics: Deep quantitative phosphoproteome analysis.

Omics: Secretome proteome analysis.

Derived from site: In situ; Brain, hippocampus; UBERON=UBERON_0002421.

Cell type: Neuron; CL=CL_0000540.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Hierarchy Parent: CVCL_U378 (HT4 [Mouse neurons])

Children:

CVCL_C0DX (HT-22 rho-0) CVCL_C0NE (HT22 Klf13 KO) CVCL_C0NF (HT22 Klf9 KO)

CVCL_C0UA (HT22 TR/TO-rKlf9 clone 2.1) CVCL_C0UB (HT22 TR/TO-mKlf13) CVCL_C0U8 (HT22-BLRP-hTRb/pSV40-BirAV5)

CVCL_C0U9 (HT22-FLBIO-rKLF9/SV40-BirAV5) CVCL_C0U7 (HT22-pSV40-BirAV5) CVCL_D6BT (HyCyte HT22 KO-mNlrp3)

CVCL_E2Q5 (HyCyte HT22 mSirt2_p.S368D_c.1102T>G_c.1103C>A) CVCL_D9WN (Ubigene HT22 Atg5 KO) CVCL_D9WP (Ubigene HT22 Atp7b KO)

CVCL_D9WQ (Ubigene HT22 Cbs KO) CVCL_D9WR (Ubigene HT22 Dgcr8 KO) CVCL_D9WS (Ubigene HT22 Lox KO)

CVCL_D9WT (Ubigene HT22 Prlr KO)

Category Transformed cell line

STR profile Source(s): Millipore=SCC129

 

Markers:

Mouse STR 1-1 10,11

Mouse STR 1-2 13,17

Mouse STR 2-1 9

Mouse STR 3-2 14,15

Mouse STR 4-2 18.3,19.3,20.3

Mouse STR 5-5 13,14,15

Mouse STR 6-4 15.3

Mouse STR 6-7 12

Mouse STR 7-1 29

Mouse STR 8-1 15

Mouse STR 11-2 15,17,18

Mouse STR 12-1 20

Mouse STR 13-1 16.2

Mouse STR 15-3 20.3

Mouse STR 17-2 12,13,14

Mouse STR 18-3 17,19

Mouse STR 19-2 11,12

Mouse STR X-1 25

 

Publications

PubMed=7953717; DOI=10.1016/0006-8993(94)90334-4

Davis J.B., Maher P.

Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line.

Brain Res. 652:169-173(1994)

 

PubMed=9292733; DOI=10.1016/S0896-6273(00)80953-8

Li Y., Maher P., Schubert D.

A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion.

Neuron 19:453-463(1997)

 

PubMed=19135458; DOI=10.1016/j.lfs.2008.12.008

Liu J., Li L.-X., Suo W.Z.

HT22 hippocampal neuronal cell line possesses functional cholinergic properties.

Life Sci. 84:267-271(2009)

 

PubMed=26227174; DOI=10.1002/pmic.201400623

Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

 

PubMed=33430126; DOI=10.3390/nu13010179; PMCID=PMC7826960

Erber L.N., Luo A., Gong Y., Beeson M., Tu M.-L., Tran P.V., Chen Y.

Iron deficiency reprograms phosphorylation signaling and reduces O-GlcNAc pathways in neuronal cells.

Nutrients 13:179.1-179.19(2021)