BV2细胞

BV2细胞、BV2细胞、BV2小鼠小胶质细胞

Cell line name BV-2

Synonyms BV2

Accession CVCL_0182

Resource Identification Initiative To cite this cell line use: BV-2 (RRID:CVCL_0182)

Comments Transformant: Recombinant retrovirus J2.

Omics: Secretome proteome analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Brain; UBERON=UBERON_0000955.

Cell type: Microglial cell; CL=CL_0000129.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: C57BL/6.

Hierarchy Children:

CVCL_ZJ35 (BV-2 PU.1 KO) CVCL_XD67 (BV2-EGFP) CVCL_C8UX (BV2-Luc)

CVCL_WJ28 (BV2-mCherry) CVCL_XD66 (BV2-tDT) CVCL_E1JF (HyCyte BV-2 KO-mAgo2)

CVCL_E1JG (HyCyte BV-2 KO-mCadm1) CVCL_E1JH (HyCyte BV-2 KO-mCd83) CVCL_E1JI (HyCyte BV-2 KO-mNpc1)

CVCL_E1JJ (HyCyte BV-2 KO-mTrem2) CVCL_D8EW (Ubigene BV-2 Ifngr1 KO) CVCL_D8EX (Ubigene BV-2 Lpl KO)

CVCL_D8EY (Ubigene BV-2 Plin2 KO) CVCL_D8EZ (Ubigene BV-2 Syk KO) CVCL_D8F0 (Ubigene BV-2 Usp7 KO)

Sex of cell Female

Age at sampling 1W

Category Transformed cell line

STR profile Source(s): Ubigene=YC-C035

 

Markers:

Mouse STR 4-2 20.3

Mouse STR 5-5 17

Mouse STR 6-4 17.3

Mouse STR 6-7 15

Mouse STR 12-1 16

Mouse STR 15-3 22.3,23.3,24.3

Mouse STR 18-3 16,17

Mouse STR X-1 27

 

Run an STR similarity search on this cell line

Publications

PubMed=2110186; DOI=10.1016/0165-5728(90)90073-V

Blasi E., Barluzzi R., Bocchini V., Mazzolla R., Bistoni F.

Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.

J. Neuroimmunol. 27:229-237(1990)

 

PubMed=19565166; DOI=10.14573/altex.2009.2.83

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)

 

PubMed=21961028; DOI=10.2174/1877381801002010148; PMCID=PMC3180920

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

 

PubMed=21214938; DOI=10.1186/1471-2164-12-14; PMCID=PMC3024950

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14.1-14.14(2011)

 

PubMed=22651808; DOI=10.1186/1742-2094-9-115; PMCID=PMC3407712

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflammation 9:115.1-115.8(2012)

 

PubMed=26227174; DOI=10.1002/pmic.201400623

Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

 

DOI=10.1007/s13273-019-00057-6

Mandal C., Yoon T., Park J.Y., Jung K.H., Chai Y.G.

Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells.

Mol. Cell. Toxicol. 16:51-61(2020)