J82细胞

J82/J82细胞系/J82细胞株/J82人膀胱移行细胞癌

Cell line name J82

Synonyms J-82; J 82; J82COT; J82 COT; J82 CO'T; J82/WT

Accession CVCL_0359

Resource Identification Initiative To cite this cell line use: J82 (RRID:CVCL_0359)

Comments Part of: BLA-40 bladder carcinoma cell line panel.

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: PTEN genetic alteration cell panel (ATCC TCP-1030).

Part of: UBC-40 urothelial bladder cancer cell line index.

Population: Caucasian; Swedish.

Doubling time: 18 + 5 hours (PubMed=9137463).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.

Sequence variations

Mutation; HGNC; 583; APC; Simple; p.Ser770Pro (c.2308T>C); ClinVar=VCV000140988; Zygosity=Heterozygous (Cosmic-CLP=753566; DepMap=ACH-000396).

Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro124Leu (c.371C>T); Zygosity=Heterozygous (ATCC=HTB-1; Cosmic-CLP=753566; DepMap=ACH-000396).

Mutation; HGNC; 9588; PTEN; Simple; p.Asn212fs*1 (c.635_1212del578); Zygosity=Homozygous (ATCC=HTB-1).

Mutation; HGNC; 9884; RB1; Simple; c.2107-2A>G; ClinVar=VCV000013077; Zygosity=Homozygous; Note=Splice acceptor mutation (PubMed=2594029; ATCC=HTB-1; Cosmic-CLP=753566; DepMap=ACH-000396).

Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=24035680).

Mutation; HGNC; 11998; TP53; Simple; p.Glu271Lys (c.811G>A); ClinVar=VCV000406566; Zygosity=Homozygous (PubMed=7787250; PubMed=9850064; ATCC=HTB-1).

Mutation; HGNC; 11998; TP53; Simple; p.Lys320Asn (c.960G>C); Zygosity=Heterozygous (PubMed=7787250; PubMed=9850064; ATCC=HTB-1).

HLA typing Source: PubMed=77569

Class I

HLA-A A*02,w32

HLA-B B*05,12

HLA-C C*w02,w03

 

Source: PubMed=26589293

Class I

HLA-A A*02:01,32:01

HLA-B B*44:02,51:01

HLA-C C*05:01,15:02

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.13

Native American 0

East Asian, North 2.62

East Asian, South 0

South Asian 0.42

European, North 70.61

European, South 26.21

Disease Bladder carcinoma (NCIt: C4912)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_8240 (Hu609) CVCL_4V98 (J82-NVB) CVCL_C8D4 (J82/MMC)

Originate from same individual CVCL_5H97 ! J82-EBV

Sex of cell Male

Age at sampling 58Y

Category Cancer cell line

STR profile Source(s): ATCC=HTB-1; CCRID; Cosmic-CLP=753566; KCLB=30001; PubMed=11416159; PubMed=27270441

 

Markers:

Amelogenin X,Y

CSF1PO 10,11

D2S1338 19

D3S1358 16,18

D5S818 12,13

D7S820 9,11

D8S1179 8,13

D13S317 10,12 (ATCC=HTB-1; CCRID; Cosmic-CLP=753566; KCLB=30001)

12 (PubMed=27270441)

D16S539 11,12

D18S51 10,12 (ATCC=HTB-1)

12 (PubMed=27270441)

D19S433 12,13

D21S11 30 (CCRID)

30,31 (ATCC=HTB-1; PubMed=11416159)

FGA 20,24

Penta D 9,13

Penta E 12,15

TH01 9.3

TPOX 11,12

vWA 17,18

 

Run an STR similarity search on this cell line

Publications

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

 

PubMed=864752; DOI=10.1093/jnci/58.6.1743

Marshall C.J., Franks L.M., Carbonell A.W.

Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.

J. Natl. Cancer Inst. 58:1743-1751(1977)

 

PubMed=870558; DOI=10.1177/25.4.870558

Benham F.J., Cottell D.C., Franks L.M., Wilson P.D.

Alkaline phosphatase activity in human bladder tumor cell lines.

J. Histochem. Cytochem. 25:266-274(1977)

 

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x

Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

Tissue Antigens 11:279-286(1978)

 

PubMed=571047

Fogh J.

Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.

Natl. Cancer Inst. Monogr. 49:5-9(1978)

 

PubMed=687519; DOI=10.1038/bjc.1978.164; PMCID=PMC2009694

O'Toole C.M., Price Z.H., Ohnuki Y., Unsgaard B.

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.

Br. J. Cancer 38:64-76(1978)

 

PubMed=6244232

Williams R.D.

Human urologic cancer cell lines.

Invest. Urol. 17:359-363(1980)

 

PubMed=6220172

Dracopoli N.C., Fogh J.

Polymorphic enzyme analysis of cultured human tumor cell lines.

J. Natl. Cancer Inst. 70:469-476(1983)

 

PubMed=6823318; DOI=10.1038/301429a0

O'Toole C.M., Povey S., Hepburn P.J., Franks L.M.

Identity of some human bladder cancer cell lines.

Nature 301:429-430(1983)

 

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

 

PubMed=4057431; DOI=10.1016/S0022-5347(17)47713-5

Bishai M.B., Kagawa S., Narayan K.S., Jones L.W., Kirk D.

Growth of a malignant human urothelial cell line (J82) in a serum-free medium (HMRI-1) developed previously for normal human urothelium.

J. Urol. 134:1287-1290(1985)

 

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

 

PubMed=3708594

Masters J.R.W., Hepburn P.J., Walker L., Highman W.J., Trejdosiewicz L.K., Povey S., Parkar M., Hill B.T., Riddle P.N., Franks L.M.

Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.

Cancer Res. 46:3630-3636(1986)

 

PubMed=3413074; DOI=10.1073/pnas.85.16.6042; PMCID=PMC281901

Pereira-Smith O.M., Smith J.R.

Genetic analysis of indefinite division in human cells: identification of four complementation groups.

Proc. Natl. Acad. Sci. U.S.A. 85:6042-6046(1988)