HUTU-80细胞

HuTu 80/HuTu 80细胞系/HuTu 80细胞株/HuTu 80人十二指肠腺癌

Cell line name HuTu 80

Synonyms HUTU 80; Hutu 80; HuTu-80; HUTU-80; Hutu-80; HUTU80; HuTu80; Hutu80

Accession CVCL_1301

Resource Identification Initiative To cite this cell line use: HuTu 80 (RRID:CVCL_1301)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Doubling time: 26.90 hours (PubMed=25944804); 26 hours (PubMed=25984343); 26-30 hours (CLS=300218).

Microsatellite instability: Stable (MSS) (PubMed=25926053; Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: According to ATCC the ethnicity of the donor is White, but exome analysis finds it to be mostly of African lineage.

Derived from site: In situ; Small intestine, duodenum; UBERON=UBERON_0002114.

Sequence variations

Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser37Phe (c.110C>T); ClinVar=VCV000017586; Zygosity=Heterozygous (ATCC=HTB-40).

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 77.36

Native American 0

East Asian, North 2.96

East Asian, South 0

South Asian 0

European, North 8.45

European, South 11.22

Disease Duodenal adenocarcinoma (NCIt: C7889)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_2862 (AZ-521)

Sex of cell Male

Age at sampling 53Y

Category Cancer cell line

STR profile Source(s): AddexBio=C0010001/49; ATCC=HTB-40; CCRID=1101HUM-PUMC000141; CLS=300218; Cosmic-CLP=907073; KCLB=30040; PubMed=25926053

 

Markers:

Amelogenin X,Y

CSF1PO 11,13

D2S1338 17,21

D3S1358 15,17

D5S818 12,13

D7S820 9,11

D8S1179 15

D13S317 8,11

D16S539 10,11

D18S51 14,17

D19S433 13,15.2

D21S11 31,32.2

FGA 21,23

Penta D 2.2

Penta E 12,18

TH01 7

TPOX 9,11

vWA 16,18

 

Run an STR similarity search on this cell line

Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221

Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

J. Natl. Cancer Inst. 59:221-226(1977)

 

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

 

PubMed=6256643; DOI=10.1038/288724a0

Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.

Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.

Nature 288:724-727(1980)

 

PubMed=22282976; DOI=10.1093/carcin/1.1.21

Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.

Human tumor cell strains defective in the repair of alkylation damage.

Carcinogenesis 1:21-32(1980)

 

PubMed=6220172

Dracopoli N.C., Fogh J.

Polymorphic enzyme analysis of cultured human tumor cell lines.

J. Natl. Cancer Inst. 70:469-476(1983)

 

PubMed=6825208; DOI=10.1093/carcin/4.2.199

Yarosh D.B., Foote R.S., Mitra S., Day R.S. 3rd

Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.

Carcinogenesis 4:199-205(1983)

 

PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Exp. Cell Biol. 52:361-370(1984)

 

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)