Hacat细胞

HaCaT/HaCaT细胞系/HaCaT细胞株/HaCaT人永生化角质形成细胞

Cell line name HaCaT

Synonyms HaCAT; HACAT; Hacat

Accession CVCL_0038

Resource Identification Initiative To cite this cell line use: HaCaT (RRID:CVCL_0038)

Comments Population: Caucasian.

Virology: Susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).

Doubling time: 28 hours (PubMed=32938951).

Omics: Deep antibody staining analysis.

Omics: Deep proteome analysis.

Omics: Metabolome analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: Although HaCaT is distributed by a number of sources, the only official DKFZ-approved supplier is CLS.

Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.

Cell type: Keratinocyte; CL=CL_0000312.

PubMed=9406811; DOI=10.1111/1523-1747.ep12340708

Henseleit U., Zhang J., Wanner R., Haase I., Kolde G., Rosenbach T.

Role of p53 in UVB-induced apoptosis in human HaCaT keratinocytes.

J. Invest. Dermatol. 109:722-727(1997)

 

PubMed=10350023; DOI=10.1023/A:1011945212831

Altenburger R., Kissel T.

The human keratinocyte cell line HaCaT: an in vitro cell culture model for keratinocyte testosterone metabolism.

Pharm. Res. 16:766-771(1999)

 

PubMed=10523843; DOI=10.1038/sj.onc.1202934

Boukamp P., Popp S., Bleuel K., Tomakidi E., Burkle A., Fusenig N.E.

Tumorigenic conversion of immortal human skin keratinocytes (HaCaT) by elevated temperature.

Oncogene 18:5638-5645(1999)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

 

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2; PMCID=PMC1850484

Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M.A., Garlick J.A., Fusenig N.E.

Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.

Am. J. Pathol. 159:1567-1579(2001)

 

PubMed=15389883; DOI=10.1002/jcb.20212

Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.

Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.

J. Cell. Biochem. 93:1048-1062(2004)

 

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020

Rasanen K., Vaheri A.

Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.

Exp. Cell Res. 316:1739-1747(2010)

 

PubMed=20100199; DOI=10.1111/j.1600-0625.2009.01026.x; PMCID=PMC2891305

Olaru F., Jensen L.E.

Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.

Exp. Dermatol. 19:e314-e316(2010)

 

PubMed=21185708; DOI=10.1016/j.jnutbio.2010.08.004

Serini S., Donato V., Piccioni E., Trombino S., Monego G., Toesca A., Innocenti I., Missori M., De Spirito M., Celleno L., Fasano E., Ranelletti F.O., Calviello G.

Docosahexaenoic acid reverts resistance to UV-induced apoptosis in human keratinocytes: involvement of COX-2 and HuR.

J. Nutr. Biochem. 22:874-885(2011)

 

PubMed=21269460; DOI=10.1186/1752-0509-5-17; PMCID=PMC3039570

Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.

Initial characterization of the human central proteome.

BMC Syst. Biol. 5:17.1-17.13(2011)

 

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690; PMCID=PMC3229662

Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.

Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.

Clin. Cancer Res. 17:7248-7264(2011)