NCI-H2444细胞

NCI-H2444细胞NCI-H2444细胞系NCI-H2444人非小细胞肺癌细胞

Cell line name NCI-H2444

Synonyms H2444; H-2444; NCIH244

Accession CVCL_1552

Resource Identification Initiative To cite this cell line use: NCI-H2444 (RRID:CVCL_1552)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: NCI RAS program mutant KRAS cell line panel.

Population: Caucasian.

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Lung; UBERON=UBERON_0002048.

Sequence variations

Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012583; Zygosity=Homozygous (Cosmic-CLP=1298356; DepMap=ACH-000186).

Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr236Cys (c.707A>G); ClinVar=VCV000376693; Zygosity=Homozygous (Cosmic-CLP=1298356; DepMap=ACH-000186).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*03:01,03:01

HLA-B B*07:02,07:02

HLA-C C*07:02,07:02

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0

Native American 1.22

East Asian, North 3

East Asian, South 0

South Asian 1.26

European, North 58.66

European, South 35.85

Disease Lung non-small cell carcinoma (NCIt: C2926)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling Age unspecified

Category Cancer cell line

STR profile Source(s): AddexBio=C0016038/5010; ATCC=CRL-5945; Cosmic-CLP=1298356; PubMed=25877200; PubMed=31467290

 

Markers:

Amelogenin X

CSF1PO 12

D2S1338 20 (PubMed=31467290)

20,25 (ATCC=CRL-5945)

D3S1358 14

D5S818 10

D7S820 8 (PubMed=25877200)

8,10 (AddexBio=C0016038/5010; ATCC=CRL-5945; Cosmic-CLP=1298356; PubMed=31467290)

D8S1179 11,12,13 (ATCC=CRL-5945; PubMed=25877200)

12,13 (PubMed=31467290)

D13S317 11,12

D16S539 12

D18S51 13

D19S433 16 (ATCC=CRL-5945)

16,17 (PubMed=31467290)

D21S11 28,29

FGA 25

Penta D 9,12

Penta E 5

TH01 9.3

TPOX 11

vWA 17

 

Run an STR similarity search on this cell line

Publications

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)

 

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

 

PubMed=18083107; DOI=10.1016/j.cell.2007.11.025

Rikova K., Guo A.-L., Zeng Q.-F., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y.-R., Tan Z.-P., Stokes M.P., Sullivan L., Mitchell J., Wetzel R., MacNeill J., Ren J.-M., Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D.-Q., Zhou X.-M., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.

Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.

Cell 131:1190-1203(2007)

 

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

 

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112; PMCID=PMC3567922

Byers L.A., Wang J., Nilsson M.B., Fujimoto J., Saintigny P., Yordy J.S., Giri U., Peyton M., Fan Y.-H., Diao L.-X., Masrorpour F., Shen L., Liu W.-B., Duchemann B., Tumula P., Bhardwaj V., Welsh J., Weber S., Glisson B.S., Kalhor N., Wistuba I.I., Girard L., Lippman S.M., Mills G.B., Coombes K.R., Weinstein J.N., Minna J.D., Heymach J.V.

Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.

Cancer Discov. 2:798-811(2012)

 

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

 

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878

Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Genome Med. 7:118.1-118.7(2015)