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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
本品可在2-8°C保存12个月。请避免长期接触阳光,不要冻结。
- 保质期:
12个月
- 规格:
50Tests/100Tests/100Tests×2
| 规格: | 50Tests | 产品价格: | ¥840.0 |
|---|---|---|---|
| 规格: | 100Tests | 产品价格: | ¥1320.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥2040.0 |
| Alternate Names |
L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T4
|
| Cellular Localization |
Membrane
|
| Clone No |
RM4-5
|
| Isotype |
Rat IgG2a, κ
|
| Leadtime |
咨询
|
| Background |
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.
|
| Abbre |
CD4
|
| Swissprot |
P06332
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Clonality |
Monoclonal
|
| Isotype Control |
Elab Fluor®700 Rat IgG2a, κ Isotype Control[2A3]
|
| Applications |
FCM
|
| Form |
Liquid
|
| Conjugation |
Elab Fluor®700
|
| Conjugation Information |
Elab Fluor® 700 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 719 nm (e.g., a 725/40 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
12504
|
| E-CK-A105 | 10×ACK Lysis Buffer | 500mL , 200mL , 100mL |
| E-CK-A107 | Cell Staining Buffer | 500mL , 200mL , 100mL |
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文献和实验Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
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