- ￥1380 - 3300
- 伊莱瑞特(Elabscience)
- 武汉
- E-AB-F1284M1
- 2025年08月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
本品可在2-8°C保存12个月。请避免长期接触阳光，不要冻结。
- 保质期：
12个月
- 规格：
50Tests/100Tests/100Tests×2
| 规格： | 50Tests | 产品价格： | ￥1380.0 |
|---|---|---|---|
| 规格： | 100Tests | 产品价格： | ￥2220.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥3300.0 |
| Alternate Names |
Hematopoietic progenitor cell antigen CD34
|
| Cellular Localization |
Membrane
|
| Clone No |
RAM34
|
| Isotype |
Rat IgG2a, κ
|
| Leadtime |
咨询
|
| Background |
The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.
|
| Abbre |
CD34
|
| Swissprot |
Q64314
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Clonality |
Monoclonal
|
| Isotype Control |
Elab Fluor®700 Rat IgG2a, κ Isotype Control[2A3]
|
| Applications |
FCM
|
| Form |
Liquid
|
| Conjugation |
Elab Fluor®700
|
| Conjugation Information |
Elab Fluor® 700 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 719 nm (e.g., a 725/40 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
12490
|
| E-CK-A105 | 10×ACK Lysis Buffer | 500mL , 200mL , 100mL |
| E-CK-A107 | Cell Staining Buffer | 500mL , 200mL , 100mL |
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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
) anti-hCD14-PE anti-hCD19-PE anti-hCD34-PE anti-hCD45-CyChrome Use corresponding PE-conjugated mouse IgG controls to evaluate specific labeling (see Step 19). CalPhos Mammalian
成员, 包括CD49a 、CD49b 、CD49c 、CD29 、CD104 等。④其他如CD90 、CD105 等。不表达造血细胞的表面标志, 如CD34 、CD45 、CD14 、CD3 、CD4 、CD8 等, 也不表达与人白细胞抗原(HLA ) 识别有关的共刺激分子及主要组织相容性复合物类分子如HLA-DR 抗原等。 但到目前为止, 对于MSC 的表面标志尚不完全确定，有些标志尚存在种属特异性，现将普遍认同的不同种属的MSCs 的表面抗原情况综述
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
. MATERIALS Reagents Anti-CD34 antibody-coupled magnetic beads (if transducing primary hematopoietic stem cells) Bovine serum albumin (BSA; 2% in PBS)
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