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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温运输,4℃保存,6个月有效。
- 库存:
现货
- 供应商:
鹿森生物
- 规格:
100ml
注意事项:无菌溶液。由碘化钾、硼酸、硫酸锌等组成,还含有少量钴离子、铜离子等每100ml溶液可制备20000ml培养基,配制好的1×工作液其碘化钾浓度为0.83mg/L、硼酸浓度为6.2mg/L、硫酸锌浓度为19.6mg/L
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
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