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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
【干冰】M1 TUBE (FLAG)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50ug
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文献和实验in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
Flow Cytometry Assay to Measure Internalization of GPCRs
. However, the user can probably also substitute PBS Ca-free, Mg-free plus 0.04% EDTA to lift cells.7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody (Sigma, Covance)requires Ca ions to bind the FLAG epitope
Flow Cytometry Assay to Measure Internalization of GPCR's
PBS Ca-free, Mg-free plus 0.04% EDTA to lift cells. 7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody ( Sigma , Covance )requires Ca ions to bind the FLAG epitope, you must rinse
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