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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
【干冰】pE-SUMOpro Amp Vector
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
20ug
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文献和实验Preparing Vector DNA For Transfection/Targeting
a recently created plasmid, restreak some of your original miniprep culture to obtain single colonies. I always go from single colonies on a plate. -Establish seeding cultures by picking 4 single colonies for each plasmid. Pick each colony into 3ml LB+amp
digestion and ligation. This method describes an alternative assembly method that allows for BioBricks to be assembled via fusion of PCR products. One PCR-amplified BioBrick has homology on each end with the second PCR-amplified BioBrick (vector amplified
the hygromycin phosphotransferase gene from the pIRESHYG3 plasmid (BD Biosciences, Palo Alto, CA, USA). Positive recombinants were selected on LB-agar plates supplemented with Hyg 30 μg/ml. Construction of the pSC101BADγβαA-I-SceI[amp] vector
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