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- GBS-786-1907
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immobilized Mouse IgG
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
5mlresin
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
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