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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Annexin V- Alexa Fluor® 647 Apoptosis Assay
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100assays
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文献和实验Apoptosis Measurement by Annexin V Staining
is associated with the condensed chromatin in apoptotic cells as a measure of apoptosis. It may appear as early as 3 h post the apoptosis inducing event and precedes the ability to detect actual DNA fragmentation. The annexin V assay has been widely accepted
Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay
can be detected in vitro and in vivo with fluorochrome- or biotin-labeled annexin V, a protein that binds to negatively charged phospholipids in the presence of calcium ions ( 6 , 7 ). In cells that are cultured in suspension, detection of apoptosis on the basis
in 1X annexin-binding buffer. Determine the cell density and dilute in 1X annexin-binding buffer to ~1 × 106 cells/mL, preparing a sufficient volume to have 100 µL per assay. 6. Add 5 µL Alexa Fluor® 488 annexin V (Component A) and 1 µL 100 µg/mL
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