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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Molecular Grinding Resin™ with Pestles & Tubes
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
5x0.5mlresinwith100Pestles&Tubes
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dihydrochloride Kodak #X8748 DNA PAGE % gel 3.5 5.0 8.0 12.0 20.0 30% acryl stock 11.6 16.6 26.6 40.0 66
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
neither requires spores to be lyophilized before grinding, nor liquid nitrogen or dry ice to be used during grinding as in other methods (Hamelin et al., 1995; Sun et al., 1995). Desiccated spores are mixed with diatomaceous earth in microfuge tubes, frozen
3X Flag Peptide) to each pelleted bead/complex, incubate 30-60 minutes at 4°C with gentle shaking;Centrifuge the resin for 30 seconds at 10,000 RPM, transfer the supernatants (which should now contain the ChIP/Flag eluted complexes) to fresh tubes
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