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- 2025年07月07日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
GET™ Total RNA
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50Preps
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文献和实验Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
. It is therefore prudent to investigate expression at the RNA and protein levels. This chapter will discuss the isolation of total RNA from yeast. The reader should refer to ref. (1 ) for a detailed description of the methods for preparation of ribosomal and small RNAs
DNase I Treatment Of Total RNA
（1 U/ul）1 ul 10X DNaseI bufferDEPC water to 10 ulExample of a scaled up version for a 40 ul reaction100 ug Total RNA4 ul DNase I （1 U/ul）4 ul 10X DNaseI bufferDEPC water to 40 ulPrepare your reaction and mix well.Incubate 37°C for 30 minutes. Stop
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