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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
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- 英文名：
picoLUCENT PLUS AP substrate only (Trial Size)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
10mlkit
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文献和实验AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
production. Following an 8 hour incubation, the filters are marked, removed, washed, blocked and incubated with the AP-fusion protein. Then, the filters are washed again and AP substrate (NBT/BCIP) is added indicating the positive plaques. These positive
colorimetric, chemiluminescent or fluorogenic substrate. Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the most popular enzyme labels used for this purpose, with HRP being the most versatile. As with traditional Western blotting, sensitivity
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