FAB固定液，RC61018-500ml

Methods for DNA isolation

for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor. Resuspend the cell pellets in old media and transfer to two bottles, centrifuge as before, and decant the media. The cell pellets can be frozen at -70degC at this point. 3. Resuspend

用 CD3/CD28 做好 T 细胞激活与扩增

细胞筛网置于培养皿中，取小鼠脾脏放置于筛网中，加入 5ml 达优小鼠淋巴细胞分离液，对脾脏轻轻进行研磨（注：此步操作要快，避免分离液蒸发）； 2. 把悬有脾脏细胞的的分离液立即转移至 15ml 离心管中，随后在分离液上层轻轻覆盖 500-1000μl 的 RPMI1640 培养基（保持分离液与培养基分界明显）； 3. 室温，水平转子 800g 离心 30 分钟，设置离心机为缓升缓降； 4. 离心结束后吸出淋巴细胞层（白膜层），加入 10ml RPMI1640 洗涤一遍细胞，250g 离心 10

Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation

under the same conditions. Then, the 53 ml of the culture was divided into 4X 2 liter flasks containing 1 liter of the same medium and incubated for additional 8-10 hours. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 500 ml bottle in the RC5-B