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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pLV3-U6-Selenos-sgRNA3-Cas9-EGFP-Puro Plasmid
- 库存:
100
- 供应商:
LSM BIO
- 规格:
2ug
pLV3-U6-Selenos-sgRNA3-Cas9-EGFP-Puro Plasmid
Catalog No.: PVT78815
pLV3-U6-Selenos-sgRNA3-Cas9-EGFP-Puro Plasmid
Alias:-
Host: Mammalian cells,lentivirus
Use(s): gene edit
Fragment Type: CRISPR
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling: Green
Promoter: U6
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验and homologous recombination. These tools provide researchers with new instruments that accelerateboth forward and reverse genetics efforts. Key words Genome engineering , Cas9 , CRISPR , CRISPR-Cas9 , PAM , Guide RNA , sgRNA , DNA cleavage , Mutagenesis
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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