- $260
- LSM BIO
- 进口
- PVT78686
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-NEFH-P2A-mCherry-Neo Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pCMV-NEFH-P2A-mCherry-Neo Plasmid
Catalog No.: PVT78686
pCMV-NEFH-P2A-mCherry-Neo Plasmid
Alias:NM_021076.4 ;CMT2CC/NFH
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Amp
Screening Markers: Neo/G418
Fluorescence labeling: Red
Promoter: CMV
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
性。 近日，《Nature Cell Biology》期刊在线发表了题为《通过内源性启动子驱动的 sgRNA 监测低丰度转录本和 lncRNAs 的表达》的研究论文，该研究通过内源基因的启动子驱动 sgRNA（single-guide RNAs）表达，结合 SPH-OminiCMV（CRISPR-activator Suntag-P65-HSF1 and OminiCMV-mCherry）荧光报告系统，成功实现低丰度基因和 lncRNAs(Long non-coding RNAs) 基因
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