- $260
- LSM BIO
- 进口
- PVT75915
- 2025年08月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pLV3-CMV-MICAL1-3×Myc-EF1a-CopGFP-Puro Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pLV3-CMV-MICAL1-3×Myc-EF1a-CopGFP-Puro Plasmid
Catalog No.: PVT75915
pLV3-CMV-MICAL1-3×Myc-EF1a-CopGFP-Puro Plasmid
Alias:NM_022765.4;MICAL; MICAL-1; NICAL
Host: Mammalian cells,lentivirus
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling: Green
Promoter: CMV
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验1.常用载体分类：2,常用载体元件介绍：(1) 启动子：启动子是位于结构基因5'端上游的DNA序列，能活化RNA聚合酶，使之与模板DNA准确的结合并具有转录起始的特异性。启动子主要分为广谱启动子和特异性启动子。广谱启动子为在各种组织、细胞上广泛表达的启动子；特异性启动子为在特异性组织或者细胞类型上表达的启动子。常用广谱启动子有CAG，CMV，EF1a，PGK等，还包含启动RNA的III型启动子U6和H1启动子；常用特异性启动子如成熟神经元特异性启动子hSyn，投射神经元特异性启动
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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