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- 2025年07月12日
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R221-75962-30
高性能色谱柱SH-I-624Sil系列
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• 中等极性键合交联聚亚芳基 (类似于 6% 氰丙级苯基/ 94%二甲基聚硅氧烷)。 |
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文献和实验6xHis-tagged protein purification using Qiagen Ni-NTA Column
lysate and mix gently by shaking at40℃for 60’Load the lysate-Ni-NTA mixture into a column with the bottom outlet capped (5ml per column)Remove bottom cap and collect the column flow throughWash twice with 4ml washing buffer every time for each column
6xHis-tagged protein purification using Qiagen Ni-NTA Column under Native Condit
lysate and mix gently by shaking at40℃for 60’Load the lysate-Ni-NTA mixture into a column with the bottom outlet capped (5ml per column)Remove bottom cap and collect the column flow throughWash twice with 4ml washing buffer every time for each column
SOUTHERN BLOTTING OF GENOMIC DNA
(to improve transfer of high molecular weight DNA): • Place the gel (on a plastic plate)in a UV oven. • Irradiate ca.1200 μJoules. • Destain 10-30 minutes in water (optional)and photograph. 4.Transfer to nylon membrane: • WEAR GLOVES when handling
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