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- SBS
- C12AL-50 (for 10uM*50ul)
- 2026年04月30日
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- 详细信息
- 文献和实验
- 技术资料
- CAS号：
C12AL-50 (for 10uM*50ul)
- 供应商：
北京赛百盛基因技术有限公司
Cas12a cleaves the substrate, consisting of FAM-biotin dual-labeled single-stranded DNA. The recommended working concentration is 120nM, sufficient for 200 reactions.
Cat. No.: C12AL-50 (for 10uM*50ul)
Description
Cas12a cleaves the substrate, consisting of FAM-biotin dual-labeled single-stranded DNA. The recommended working concentration is 120nM, sufficient for 200 reactions.
Specification
10uM*50ul
SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.
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文献和实验In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins
. Here we describe a generalized in vitro selection experiment aiming at the enrichment of ssDNA aptamers using biotinylated target molecules. This procedure allows the application of streptavidin–biotin chemistry to separate bound from unbound DNA species during the selection
Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA
scenario assessment important for resource mobilization and threat confinement. Lateral-flow assays (LFAs) and dip-stick assays, which are typically antibody-based and yield a visually detectable signal, provide an assay format suiting these applications
。 Target 设计 Target 设计：通常情况下我们仅需要在你的目的位置附近找到 NGG（N 代表任意核苷酸），然后找到其紧邻的上游 20 nt 即可（如上图）。 目的位置的选择：我们绝大部分时候的目的是完全 KO 一个基因，其原理是利用移码突变（CRISPR/Cas 系统诱导产生 indel）。所以我们一般选择距离起始密码子 ATG 下游 200bp 范围内的 exon 区域。另外，通常一个基因往往会转录成 2 个以上的 isoforms，所以请选择尽量
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