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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 供应商:
联硕生物
- 规格:
500mL
与其他基础培养基相比,F-12 含有更广泛的成分,包括锌、腐胺、次黄嘌呤和胸苷。CHO 细胞在 F-12 中的无血清生长催生了各种改良配方。含有 GlutaMAX™ 补充剂的 Gibco™ F-12 培养基具有易于使用的包装形式,可显著减少有毒氨的积累,改善细胞活力和生长。
使用 Ham's F-12 营养混合物 Gibco™ F-12 不含蛋白或生长因子。因此,F-12 需要添加剂,通常采用 10%胎牛血清 (FBS)。F-12 使用碳酸氢钠缓冲系统 (1.176 g/L),因此需要 5-10% CO2 环境来维持生理 pH 值。
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文献和实验Kamps's Western Blotting Protocol
to 30 min in 3% BSA in rinse buffer .This should be filtered prior to use.In Mark Kamps's original procedure,he used 5% BSA and 1% ovalbumin.3% BSA appears to work just as well.1% BSA may also work satisfactorily,but you may be pushing your luck
Raft culture media (aka Green’s media)
Raft culture media (aka Green’s media) 3 parts DMEM (including glutamine or glutamax) 1 part Ham’s F12 5% FCS various supplements (not everybody uses the same supplements, some don’t use
Protocols for the preparation of tumour cells for s.c. injections in mice
. The trypsinized cells are transferred into a 15 ml falcon tube and 10 ml medium added. After centrifugation (5 min., 1100 rpm), cells are resuspended in sterile PBS (12 ml) and centrifuged again. The cell pellet is then resuspended in 500-600 µl of PBS and kept
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