J.gamma1人急性T细胞白血病细胞系
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J.gamma1人急性T细胞白血病细胞系

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  • J.gamma1人急性T细胞白血病细胞系
  • 美国、德国、欧洲等
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

      详见细胞说明资料

    • 细胞类型

      详见细胞说明资料

    • 肿瘤类型

      详见细胞说明资料

    • 供应商

      上海冠导生物工程有限公司

    • 库存

      ≥100瓶

    • 生长状态

      详见细胞说明资料

    • 年限

      详见细胞说明资料

    • 运输方式

      常温运输【复苏细胞】或干冰运输【冻存细胞】

    • 器官来源

      详见细胞说明资料

    • 是否是肿瘤细胞

      详见细胞说明资料

    • 细胞形态

      详见细胞说明资料

    • 免疫类型

      详见细胞说明资料

    • 物种来源

      详见细胞说明资料

    • 相关疾病

      详见细胞说明资料

    • 组织来源

      详见细胞说明资料

    • 英文名

      J.gamma1人急性T细胞白血病细胞系

    • 规格

      1*10(6)Cellls/瓶

    "J.gamma1人急性T细胞白血病细胞系
    传代比例:1:2-1:4(首次传代建议1:2)
    生长特性:悬浮生长
    换液周期:每周2-3次
    冻存和复苏的原则:慢冻快融》当细胞冷到零度以下,可以产生以下变化:细胞器脱水,细胞中可溶性物质浓度升GAO,并在细胞内形成冰晶。如果缓慢冷冻,可使细胞逐步脱水,细胞内不致产生大的冰晶;相反,结晶就大,大结晶会造成细胞膜、纲胞器的损伤和破裂。复苏过程应快融,目的是防止小冰晶形成大冰晶,即冰晶的重结晶。慢冻程序》1.标准程序:采用细胞冻存器》当温度在-25℃以上时,1~2℃/min;当温度达-25℃以下时,5~10℃/min;当温度达-100℃时,可迅速放入中。2.简易程序:将冷冻管(管口要朝上)放入纱布袋内,纱布袋系以线绳,通过线绳将纱布袋固定于罐罐口,按每分钟温度下降1~2℃的速度,在40min内降至表面过夜,次晨投人中。3.传统程序:冷冻管置于4℃10分钟→-20℃30分钟→-80℃16~18小时(或隔夜)→槽长期储存。细胞冻存方法:1.预先配制冻存》(1)8%DMSO+细胞生长(92%血清)2.取对数生长期细胞,经胰酶消化后,加入适量冻存,用吸管吹打制成细胞悬(1×10(6)~5×10(6)细胞/ml)。3.加入1ml细胞于冻存管中,密封后标记冷冻细胞名称和冷冻日期。长期保存。保存细胞的复苏方法:1.快速解冻》冻存细胞从中取出后,立即放入37℃水浴中,轻轻摇动冷冻管,使其在1分钟内全部融化(不要超过3分钟)。2.解冻后的细胞可直接接种到含完全生长培养的细胞培养瓶中直接进行培养,24小时后再用新鲜完全培养替换旧培养,以去除DMSO。3.如果细胞对冷冻保护剂别敏感解冻后的细胞应先通过离心去除冷冻保护剂,然后再接种到含完全生长培养的培养瓶中。
    J.gamma1人急性T细胞白血病细胞系
    背景信息:详见相关文献介绍
    ┈订┈购┈热┈线:1┈5┈8┈0┈0┈5┈7┈6┈8┈6┈7【微信同号】┈Q┈Q:3┈3┈0┈7┈2┈0┈4┈2┈7┈1;
    MDAMB435 Cells;背景说明:乳腺癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:NCIH647 Cells、PTK1 Cells、UACC 812 Cells
    CA-OV-3 Cells;背景说明:该细胞1976年建系,源自一位54岁白人女性的卵巢腺癌组织。;传代方法:1:3传代,2—3天换液一次;生长特性:贴壁生长;形态特性:上皮样;相关产品有:HIT T15 Cells、VK2 (E6/E7) Cells、L6565 Cells
    95D Cells;背景说明:这是一株高转移肺癌。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:OCUM-1 Cells、C4-2 Cells、NTHY-ORI3.1 Cells
    RMS 1598 Cells;背景说明:详见相关文献介绍;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:PC 61.5.3 Cells、JJN-3 Cells、NCIH1693 Cells
    产品包装:复苏发货:T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)
    来源说明:细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库
    J.gamma1人急性T细胞白血病细胞系
    细胞培养相关问题总结:冷冻管应如何解冻?取出冷冻管后,须立即放入37°C水槽中快速解冻,轻摇冷冻管使其在1分钟内全部融化,并注意水面不可超过冷冻管盖沿,否则易发生污染情形。另冷冻管由桶中取出解冻时,必须注意安全,预防冷冻管之爆裂;细胞冷冻管解冻培养时,是否应马上去除DMSO?除少数别注明对DMSO敏感之细胞外,绝大部分细胞株(包括悬浮性细胞),在解冻之后,应直接放入含有10-15ml新鲜培养基之培养角瓶中,待隔天再置换新鲜培养基以去除DMSO 即可,如此可避免大部分解冻后细胞无法生长或贴附之问题;可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其定使用且已适应之细胞培养基,若骤然使用和原先提供之培养条件不同之培养基,细胞大都无法立即适应,造成细胞无法存活;可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生大的影响。来自不同物种的血清,在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活;培养细胞时应使用5%或10%CO2?或根本没有影响?一般培养基中大都使用HCO3-/CO32-/H+作为pH的缓冲系统,而培养基中NaHCO3的含量将决定细胞培养时应使用的CO2浓度。当培养基中NaHCO3含量为每公升3.7g时,细胞培养时应使用10%CO2;当培养基中NaHCO3为每公升1.5g时,则应使用5%CO2培养细胞。何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。培养基中是否须添加抗生素?除于殊筛选系统中外,一般正常培养状态下,培养基中不应添加任何抗生素。
    SNU761 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:NCIH1694 Cells、H1755 Cells、Bac 1.2F5 Cells
    GM03573A Cells;背景说明:这是P3X63Ag8(ATCCTIB-9)的一个不分泌克隆。Kappa链合成了但不分泌。能抗0.1mM8-氮杂鸟嘌呤但不能在HAT培养基中生长。据报道它是由于缺失了3-酮类固醇还原酶活性的胆固醇营养缺陷型。检测表明肢骨发育畸形病毒(鼠痘)阴性。;传代方法:1:2传代,3天内可长满。;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:Panc 5.04 Cells、NOMO1 Cells、LLC-PK1 Cells
    NUGC2 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:SuDHL 1 Cells、GM05887A Cells、P3/X63/Ag8.653 Cells
    物种来源:人源、鼠源等其它物种来源
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    形态特性:淋巴母细胞样
    目前,跟公司签订长期战略合作伙伴的科研单位有山西中医学院、福建医科大学附属第二医院、上海市第六人民医院、北京大学医学部、同济大学、四平中心医院、广州中医药大学、中科院上海生命科学研究院生化与细胞研究所、解放军第306医院、江南大学、西南医院、吉首大学、西南大学化学化工学院、宁波大学、福建医科大学附属第一医院、苏州市同济医院、中南大学、延安大学附属医院、中国科学院上海药物研究所、天津市儿童医院、江苏省中医药研究院、湘雅医学院、第四军医大学预防系劳动与环境教研室、扬州大学、上海交通大学、中国医科大学、浙江省立同德医院、中国人民解放军第150中心医院、苏州大学、四川大学华西医院、云南大学、扬州大学兽医学院、上海交通大学医学院附属第九人民医院、海南医学院、江苏肿瘤医院、上海市闵行区中心医院、大连医科大学附属第一医院、中国科学院广州生物医药与健康研究院、沈阳药科大学、广州市第八人民医院、南京大学、浙江中医药大学、兰州大学、上海中医药大学、中国农业科学院饲料研究所、厦门市妇幼保健院、大学、南京军区总医院、天津市人民医院、北京医学科学肿瘤医院、上海市同济医院、江苏大学、桂林医学院附属医院、中国科学院化学研究所
    MC26 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:Hs-274-T Cells、TW 01 Cells、HC-11 Cells
    EFM-19 Cells;背景说明:乳腺管癌;胸腔积液转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:SKMES Cells、RD-ES Cells、HT-115 Cells
    KYSE-270 Cells;背景说明:详见相关文献介绍;传代方法:1:5传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MCA 38 Cells、NCI-H2108 Cells、GM02132C Cells
    MDAMB231 Cells;背景说明:MDA-MB-231来自患有转移乳腺腺癌的51岁女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中,它能形成低分化腺癌(III级)。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MUTZ-1 Cells、PANC-08-13 Cells、H128 Cells
    J 82 Cells;背景说明:电子显微镜下未观察到桥粒但观察到数目不同的粗面内质网和突出微丝。 含ras (H-ras)癌基因。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:RK-13 Cells、MNNG-HOS (TE 85, clone F-5) Cells、REC Cells
    NCI-H2110 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代,每周换液2-3次;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:上皮细胞;相关产品有:E6-1 Cells、G-292 clone A141B1 Cells、Fukuoka University-Malignant mixed Mullerian Tumor-1 Cells
    L 428 Cells;背景说明:霍奇金淋巴瘤;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:SNU475 Cells、HCC-1833 Cells、QGP1 Cells
    HEK 293-EBNA Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:10传代;每周2次。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HIT clone T15 Cells、NCIH211 Cells、SUM-52-PE Cells
    NCI-H2291 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:4传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:NCI-H1299 Cells、OCI-LY-19 Cells、H1435 Cells
    SW1417 Cells;背景说明:详见相关文献介绍;传代方法:1:2—1:4传代,每周换液1-2次;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:B-16 Cells、Kuramochi Cells、L Cells
    P815 Cells;背景说明:肥大细胞瘤;雄性;DBA/2;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:SKMEL2 Cells、NPA'87 Cells、hFOB 1.19 Cells
    H125 Cells;背景说明:腺鳞状肺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:MOLM16 Cells、LLC-WRC 256 Cells、RPPVEC Cells
    NCI-H1623 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2-3次。;生长特性:贴壁生长;形态特性:详见产品说明;相关产品有:COLO 320HSR Cells、HHUA Cells、ST486 Cells
    OEC33 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:ZR-75-1 Cells、HPF Cells、Wien133 Cells
    Tb 1 Lu Cells;背景说明:肺;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:MCF.10A Cells、BEL 7402 Cells、NHRF Cells
    J.gamma1人急性T细胞白血病细胞系
    NCTC-1469 Cells;背景说明:1952年建系,源于正常C3H/An小鼠的肝脏组织,表达H-2K抗原,鼠痘病毒阴性。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:淋巴母细胞;相关产品有:COLO-357 Cells、Jeko1 Cells、FRhK4 Cells
    H1092 Cells;背景说明:详见相关文献介绍;传代方法:随细胞的密度而增加;生长特性:悬浮生长;形态特性:详见产品说明;相关产品有:MM134 Cells、C166 Cells、MCF/Adr Cells
    Abcam HEK293T HK1 KO Cells(拥有STR基因鉴定图谱)
    AG10643 Cells(拥有STR基因鉴定图谱)
    BayGenomics ES cell line PST100 Cells(拥有STR基因鉴定图谱)
    BayGenomics ES cell line XC819 Cells(拥有STR基因鉴定图谱)
    BTIC Cells(拥有STR基因鉴定图谱)
    CpHi28 Cells(拥有STR基因鉴定图谱)
    ┈订┈购┈热┈线:1┈5┈8┈0┈0┈5┈7┈6┈8┈6┈7【微信同号】┈Q┈Q:3┈3┈0┈7┈2┈0┈4┈2┈7┈1;
    DA04158 Cells(拥有STR基因鉴定图谱)
    DFSP Cells(拥有STR基因鉴定图谱)
    GM02690 Cells(拥有STR基因鉴定图谱)
    B-104 Cells;背景说明:神经母细胞瘤;BDIX;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:COLO-320DM Cells、SaOS Cells、RMS 13 Cells
    Rh30 Cells;背景说明:肺泡横纹肌肉瘤;骨髓转移;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:HT 115 Cells、Ramos.G6.C10 Cells、JM-Jurkat Cells
    HOS TE 85 Cells;背景说明:骨肉瘤;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:293AD Cells、LI7 Cells、MOVAS-1 Cells
    TE353.SK Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:详见产品说明;相关产品有:Calu-3 Cells、P3/NSI/1-AG4-1 Cells、BC-021 Cells
    639-V Cells;背景说明:膀胱癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:PA-TU-8988S Cells、CAL27 Cells、NCI-H1435 Cells
    MARC-145 Cells;背景说明:胚肾;自发永生;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:MC-26 Cells、HUVEC Cells、HOMEC Cells
    MOLT-16 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:T-cell Acute Lymphoblastic Leukemia-1 Cells、HT 144 Cells、NCIH1437 Cells
    H2023 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2次;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:SUPT-1 Cells、Hmy.2 CIR Cells、H1651 Cells
    OV3121 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:BCP-1 Cells、NCI-BL1339 Cells、Hs294 Cells
    AR4IP Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代;每周2-3次。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:D341 Med Cells、IEC 6 Cells、SUDHL-16 Cells
    CAL148 Cells;背景说明:乳腺癌;胸腔积液转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:Hs895T Cells、QG-56 Cells、SKOV-433 Cells
    HFF Cells;背景说明:详见相关文献介绍;传代方法:1:5—1:7传代;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:MAEC Cells、HuH 6 Cells、P3.653 Cells
    8305C Cells;背景说明:详见相关文献介绍;传代方法:1:6传代;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:MKN28 Cells、NCI-H719 Cells、SK-MEL2 Cells
    4G7 Cells(拥有STR基因鉴定图谱)
    Laboratory of Allergic Diseases 2 Cells;背景说明:肥大 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:SU-DHL-1 Cells、Hep 3B2_1-7 Cells、GM-215 Cells
    HOP-92 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:L-WRN Cells、SCC25 Cells、LA-N-6 Cells
    NT2-D1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:Lewis Lung Cells、SK-MES Cells、A253 Cells
    67NR Cells;背景说明:乳腺癌;BALB/cfC3H;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:Hs839T Cells、H2195 Cells、LLCMK2 Cells
    MCF 7 Cells;背景说明:MCF-7细胞保留了多个分化了的乳腺上皮的特性,包括:能通过胞质雌激素受体加工雌二醇并能形成圆形复合物(domes)。该细胞含有Tx-4癌基因。肿瘤坏死因子α(TNFalpha)可以抑制MCF-7细胞的生长。抗雌激素处理细胞能调变IGFBP'S的分泌。;传代方法:1:2传代,3-4天长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:293-GP Cells、MyLa2059 Cells、NCI-H2444 Cells
    CCRF-CEM Cells;背景说明:G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。此细胞系从香港收集而来。;传代方法:1:2传代。3天内可长满。;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:SNU-601 Cells、ZR-75-1 Cells、OCLY8 Cells
    SKN-AS Cells;背景说明:详见相关文献介绍;传代方法:1:5-1:10传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:NCI-H1238 Cells、Glioma-261 Cells、Earle's L cells Cells
    RPMI1788 Cells;背景说明:B淋巴细胞;EBV转化;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:NCI-H1436 Cells、UM-UC14 Cells、NCI-H1436 Cells
    GM11424 Cells(拥有STR基因鉴定图谱)
    HAP1 CTNNA1 (-) 1 Cells(拥有STR基因鉴定图谱)
    EBTr (NBL-4) Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:Hep3B Cells、MD Anderson-Metastatic Breast-134-VI Cells、HHL5 Cells
    HGFDFSV40T168 Cells(拥有STR基因鉴定图谱)
    INPERe002-A Cells(拥有STR基因鉴定图谱)
    M21 Cells(拥有STR基因鉴定图谱)
    ND05952 Cells(拥有STR基因鉴定图谱)
    PENN097i-17-1 Cells(拥有STR基因鉴定图谱)
    Ubigene HEK293 ATG7 KO Cells(拥有STR基因鉴定图谱)
    WCMC-23 Cells(拥有STR基因鉴定图谱)
    HAP1 TMEM251 (-) Cells(拥有STR基因鉴定图谱)
    Wistar Institute-38 Cells;背景说明:LeonardHayflick建系;有限传代细胞系;寿命为50±10代(倍增时间24h);来自妊娠3个月的正常胚胎肺组织。该细胞系是第一个用于人制备的人二倍体细胞;培养基中添加TNFα可以加快细胞生长。;传代方法:1:2-1:4传代;2-3天换液1次;生长特性:贴壁生长;形态特性:成纤维细胞样;相关产品有:PK(15) Cells、WM115 Cells、PC 61-5-3 Cells
    EFM-192B Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:NCIH548 Cells、CEK Cells、Hs 578T Cells
    Lieming Xu-2 Cells;背景说明:详见相关文献介绍;传代方法:1:3传代,2-3天换液一次;生长特性:贴壁生长;形态特性:详见产品说明;相关产品有:HSC Cells、H810 Cells、U251n Cells
    CCD-841-CoTr Cells;背景说明:结肠癌;SV40转化;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:MEF (CF-1) Cells、LS513 Cells、HCoEpiC Cells
    MDA-MB-435S Cells;背景说明:MDA-MB-435S是一种纺锤形的细胞,1976年由其亲本(435)中筛选得到。435是从31岁的转移性乳腺导管腺癌女性患者胸水中分离得到。当用荧光染料对微管蛋白进行染色时亲本细胞显现散布特征(II型)。最近通过cDNA阵列研究表明,亲本(MDA-MB-435)可归入黑素瘤起源。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:纺锤形;相关产品有:Hs_578t Cells、EoL-1 cell Cells、G402 Cells
    MDA-MB-435S Cells;背景说明:MDA-MB-435S是一种纺锤形的细胞,1976年由其亲本(435)中筛选得到。435是从31岁的转移性乳腺导管腺癌女性患者胸水中分离得到。当用荧光染料对微管蛋白进行染色时亲本细胞显现散布特征(II型)。最近通过cDNA阵列研究表明,亲本(MDA-MB-435)可归入黑素瘤起源。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:纺锤形;相关产品有:Hs_578t Cells、EoL-1 cell Cells、G402 Cells
    PK(15) Cells;背景说明:PK-15细胞建系于1955(Stice,E)。是PK-1a细胞的克隆系。该细胞系可用于多种病毒的增值及特性研究。另外,电镜观察发现,PK-15细胞内有C-型病毒颗粒存在,是研究C-型病毒的材料。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:上皮细胞样;相关产品有:SW403 Cells、T/G HA-VSMC Cells、BGC823 Cells
    CL-11 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:University of Michigan-Urothelial Carcinoma-1 Cells、MGH-U3 (RN) Cells、RC13 Cells
    NCI-H2066 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2-3次;生长特性:悬浮生长;形态特性:聚团悬浮;相关产品有:Panc_08_13 Cells、Hs606T Cells、3T3 Cells
    N1E-115 Cells;背景说明:神经母细胞瘤;雄性;A/J;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:87MG Cells、MDA-MB-175-VII Cells、AML193 Cells
    MDAMB435S Cells;背景说明:MDA-MB-435S是一种纺锤形的细胞,1976年由其亲本(435)中筛选得到。435是从31岁的转移性乳腺导管腺癌女性患者胸水中分离得到。当用荧光染料对微管蛋白进行染色时亲本细胞显现散布特征(II型)。最近通过cDNA阵列研究表明,亲本(MDA-MB-435)可归入黑素瘤起源。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:纺锤形;相关产品有:Caco-2/BBe 1 Cells、TOV21 Cells、NCI-SNU-520 Cells
    MDAMB330 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:Sarcoma OSteogenic-2 Cells、OsA-CL Cells、MADISON LUNG TA-109 Cells
    207 Cells;背景说明:儿童急性髓系白血病;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:CHOS Cells、Panc-03.27 Cells、HEC1-A Cells
    BJA-B Cells;背景说明:Burkitt's淋巴瘤;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:Y3Ag1.2.3 Cells、NE-1 Cells、BC-020 Cells
    SNB.19 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明;相关产品有:LLC-MK2 Cells、Alpha Mouse Liver 12 Cells、KYSE-180 Cells
    SDUBMSi001-A Cells(拥有STR基因鉴定图谱)
    MCA 205 Cells;背景说明:纤维肉瘤;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:SW 1417 Cells、Vero-E6 Cells、SCL1 Cells
    Hs852T Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:UCLA NPA871 Cells、NCI-H1573 Cells、KTA-7 Cells
    SUP-M2 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:SW1271 Cells、D283MED Cells、Baby Hamster Kidney-21 Cells
    Ontario Cancer Institute-Acute Myeloid Leukemia-2 Cells;背景说明:急性髓系白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明;相关产品有:HFB Cells、PL-5 Cells、MCF 7B Cells
    SUDHL-6 Cells;背景说明:详见相关文献介绍;传代方法:1:3—1:6传代,3—4天换液1次;生长特性:悬浮生长 ;形态特性:淋巴母细胞样;相关产品有:MCF-10 Attached Cells、University of Michigan-Renal Carcinoma-2 Cells、RGC-5 Cells
    HuH1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:NCI-H747 Cells、Wien133 Cells、AKR Cells
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    YES2 Cells;背景说明:食管鳞癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:MDAMB175 Cells、WM2664 Cells、H-295R Cells
    T98-G Cells;背景说明:详见相关文献介绍;传代方法:按1:3传代;生长特性:贴壁生长;形态特性:详见产品说明;相关产品有:H-28 Cells、D341 Med Cells、OV1/P Cells
    Human Kidney-2 Cells;背景说明:该细胞属源于正常肾的近曲小管细胞,通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2,Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317,最后将PA317产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性,但未用G418筛选转导克隆。Southern和FISH分析显示HK-2细胞来源于单克隆。PCR检测证实HK-2细胞基因组中含有E6/E7基因。;传代方法:1:4传代;2-3天换液1次;生长特性:贴壁生长;形态特性:上皮样;相关产品有:NCI-H1975 Cells、B16-F10 Cells、H-1876 Cells
    HEK-293F Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;悬浮生长;形态特性:上皮细胞样;相关产品有:Jurkat E6 Cells、MDA-435 Cells、Hs 940.T Cells
    HEP-3B2 Cells;背景说明:肝癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:CNE-2Z Cells、MV-3 Cells、HCS2/8 Cells
    Eca 109 Cells;背景说明:1973年建系,来自人食管中段鳞癌组织,小块法原代培养建系。BALB/c裸鼠移植成瘤。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:上皮细胞样;相关产品有:HO8910/PM Cells、H3255_DA Cells、H-87 Cells
    NCI-H676B Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明部分;形态特性:详见产品说明;相关产品有:SNU-216 Cells、COV362 Cells、ID8 Cells
    J.gamma1人急性T细胞白血病细胞系
    QGP1 Cells;背景说明:胰腺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明;相关产品有:IM 9 Cells、U 937 Cells、ECV Cells
    BayGenomics ES cell line RRK213 Cells(拥有STR基因鉴定图谱)
    BayGenomics ES cell line XL817 Cells(拥有STR基因鉴定图谱)
    GIBH001 Cells(拥有STR基因鉴定图谱)
    OHP7D7.2.3 Cells(拥有STR基因鉴定图谱)
    ZH68-2A4 Cells(拥有STR基因鉴定图谱)
    MCW047i-U2234 Cells(拥有STR基因鉴定图谱)
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    Naito S., Inoue S., Kinjo M., Tanaka K.
    Thromboplastic and fibrinolytic activities of cultured human gastric cancer cell lines.
    Gann 74:240-247(1983)

    PubMed=3986962; DOI=10.1093/carcin/6.4.549
    Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-i., Mori T., Kondo S.
    Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.
    Carcinogenesis 6:549-553(1985)

    PubMed=3962675; DOI=10.1111/j.1440-1827.1986.tb01461.x
    Motoyama T., Hojo H., Watanabe H.
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    Acta Pathol. Jpn. 36:65-83(1986)

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    Watson S.A., Durrant L.G., Morris D.L.
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    Int. J. Cancer 45:90-94(1990)

    PubMed=1778766; DOI=10.1111/j.1349-7006.1991.tb01816.x; PMCID=PMC5918361
    Takeshima E., Hamaguchi M., Watanabe T., Akiyama S., Kataoka M., Ohnishi Y., Xiao H.-Y., Nagai Y., Takagi H.
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    Jpn. J. Cancer Res. 82:1428-1435(1991)

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    Durrant L.G., Watson S.A., Hall A., Morris D.L.
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    Br. J. Cancer 63:67-70(1991)

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    Yamada Y., Yoshida T., Hayashi K., Sekiya T., Yokota J., Hirohashi S., Nakatani K., Nakano H., Sugimura T., Terada M.
    p53 gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases.
    Cancer Res. 51:5800-5805(1991)

    PubMed=2061947; DOI=10.1093/jnci/83.12.866
    Watson S.A., Durrant L.G., Wencyk P.M., Watson A.L., Morris D.L.
    Intracellular gastrin in human gastrointestinal tumor cells.
    J. Natl. Cancer Inst. 83:866-871(1991)

    PubMed=1370612; DOI=10.1016/S0006-291X(05)80133-0
    Matozaki T., Sakamoto C., Matsuda K., Suzuki T., Konda Y., Nakano O., Wada K., Uchida T., Nishisaki H., Nagao M., Kasuga M.
    Missense mutations and a deletion of the p53 gene in human gastric cancer.
    Biochem. Biophys. Res. Commun. 182:215-223(1992)

    PubMed=1486568; DOI=10.1016/0165-4608(92)90350-H
    Rege-Cambrin G., Scaravaglio P., Carozzi F., Giordano S., Ponzetto C., Comoglio P.M., Saglio G.
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    Cancer Genet. Cytogenet. 64:170-173(1992)

    DOI=10.1016/B978-0-12-333530-2.50014-9
    Sekiguchi M., Suzuki T.
    Gastric tumor cell lines.
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    PubMed=9023415; DOI=10.1006/cimm.1996.1062
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    Hatakeyama S., Gao Y.-H., Ohara-Nemoto Y., Kataoka H., Satoh M.
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    Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
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    PubMed=9665481; DOI=10.1016/S0002-9440(10)65561-7; PMCID=PMC1852940
    Paciucci R., Vila M.R., Adell T., Diaz V.M., Tora M., Nakamura T., Real F.X.
    Activation of the urokinase plasminogen activator/urokinase plasminogen activator receptor system and redistribution of E-cadherin are associated with hepatocyte growth factor-induced motility of pancreas tumor cells overexpressing Met.
    Am. J. Pathol. 153:201-212(1998)

    PubMed=11107048; DOI=10.1046/j.1440-1827.2000.01117.x
    Yokozaki H.
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    Pathol. Int. 50:767-777(2000)

    PubMed=11314020; DOI=10.1038/sj.onc.1204160
    Kataoka H., Miura Y., Joh T., Seno K., Tada T., Tamaoki T., Nakabayashi H., Kawaguchi M., Asai K., Kato T., Itoh M.
    Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1.
    Oncogene 20:869-873(2001)

    PubMed=11668190; DOI=10.1177/002215540104901105
    Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
    Immunocytochemical analysis of cell lines derived from solid tumors.
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    PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x; PMCID=PMC11160020
    Takada H., Imoto I., Tsuda H., Sonoda I., Ichikura T., Mochizuki H., Okanoue T., Inazawa J.
    Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
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    PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
    Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
    Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
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    PubMed=15900046; DOI=10.1093/jnci/dji133
    Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
    p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
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    PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
    Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
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    Pathol. Res. Pract. 201:109-115(2005)

    PubMed=18804159; DOI=10.1016/j.ygeno.2008.08.002
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    Next-generation characterization of the Cancer Cell Line Encyclopedia.
    Nature 569:503-508(2019)"

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    该产品被引用文献
    "PubMed=6862145; DOI=10.20772/cancersci1959.74.2_240
    Naito S., Inoue S., Kinjo M., Tanaka K.
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    Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-i., Mori T., Kondo S.
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    Carcinogenesis 6:549-553(1985)

    PubMed=3962675; DOI=10.1111/j.1440-1827.1986.tb01461.x
    Motoyama T., Hojo H., Watanabe H.
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    Acta Pathol. Jpn. 36:65-83(1986)

    PubMed=2105279; DOI=10.1002/ijc.2910450117
    Watson S.A., Durrant L.G., Morris D.L.
    The effect of the E2 prostaglandin enprostil, and the somatostatin analogue SMS 201 995, on the growth of a human gastric cell line, MKN45G.
    Int. J. Cancer 45:90-94(1990)

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    Jpn. J. Cancer Res. 82:1428-1435(1991)

    PubMed=1846553; DOI=10.1038/bjc.1991.14; PMCID=PMC1971664
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    Br. J. Cancer 63:67-70(1991)

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    Cancer Res. 51:5800-5805(1991)

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    Watson S.A., Durrant L.G., Wencyk P.M., Watson A.L., Morris D.L.
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    Matozaki T., Sakamoto C., Matsuda K., Suzuki T., Konda Y., Nakano O., Wada K., Uchida T., Nishisaki H., Nagao M., Kasuga M.
    Missense mutations and a deletion of the p53 gene in human gastric cancer.
    Biochem. Biophys. Res. Commun. 182:215-223(1992)

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    Cancer Genet. Cytogenet. 64:170-173(1992)

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    Sekiguchi M., Suzuki T.
    Gastric tumor cell lines.
    (In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.287-316; Academic Press; New York; USA (1994)

    PubMed=9023415; DOI=10.1006/cimm.1996.1062
    Seki N., Hoshino T., Kikuchi M., Hayashi A., Itoh K.
    HLA-A locus-restricted and tumor-specific CTLs in tumor-infiltrating lymphocytes of patients with non-small cell lung cancer.
    Cell. Immunol. 175:101-110(1997)

    PubMed=9247707; DOI=10.1080/15216549700202901
    Hatakeyama S., Gao Y.-H., Ohara-Nemoto Y., Kataoka H., Satoh M.
    Expression of bone morphogenetic proteins of human neoplastic epithelial cells.
    Biochem. Mol. Biol. Int. 42:497-505(1997)

    PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
    Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
    Screening the p53 status of human cell lines using a yeast functional assay.
    Mol. Carcinog. 19:243-253(1997)

    PubMed=9665481; DOI=10.1016/S0002-9440(10)65561-7; PMCID=PMC1852940
    Paciucci R., Vila M.R., Adell T., Diaz V.M., Tora M., Nakamura T., Real F.X.
    Activation of the urokinase plasminogen activator/urokinase plasminogen activator receptor system and redistribution of E-cadherin are associated with hepatocyte growth factor-induced motility of pancreas tumor cells overexpressing Met.
    Am. J. Pathol. 153:201-212(1998)

    PubMed=11107048; DOI=10.1046/j.1440-1827.2000.01117.x
    Yokozaki H.
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    Pathol. Int. 50:767-777(2000)

    PubMed=11314020; DOI=10.1038/sj.onc.1204160
    Kataoka H., Miura Y., Joh T., Seno K., Tada T., Tamaoki T., Nakabayashi H., Kawaguchi M., Asai K., Kato T., Itoh M.
    Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1.
    Oncogene 20:869-873(2001)

    PubMed=11668190; DOI=10.1177/002215540104901105
    Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
    Immunocytochemical analysis of cell lines derived from solid tumors.
    J. Histochem. Cytochem. 49:1369-1378(2001)

    PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x; PMCID=PMC11160020
    Takada H., Imoto I., Tsuda H., Sonoda I., Ichikura T., Mochizuki H., Okanoue T., Inazawa J.
    Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
    Cancer Sci. 96:100-110(2005)

    PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
    Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
    Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
    Mol. Cancer Ther. 4:399-412(2005)

    PubMed=15900046; DOI=10.1093/jnci/dji133
    Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
    p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
    J. Natl. Cancer Inst. 97:765-777(2005)

    PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
    Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
    Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
    Pathol. Res. Pract. 201:109-115(2005)

    PubMed=18804159; DOI=10.1016/j.ygeno.2008.08.002
    Jung J.-J., Jeung H.-C., Chung H.C., Lee J.O., Kim T.S., Kim Y.T., Noh S.H., Rha S.Y.
    In vitro pharmacogenomic database and chemosensitivity predictive genes in gastric cancer.
    Genomics 93:52-61(2009)

    PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
    Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
    Signatures of mutation and selection in the cancer genome.
    Nature 463:893-898(2010)

    PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
    Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
    A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
    Cancer Res. 70:2158-2164(2010)

    PubMed=22531121; DOI=10.2739/kurumemedj.58.73
    Koga A., Aoyagi K., Imaizumi T., Miyagi M., Shirouzu K.
    Comparison between the gastric cancer cell line MKN-45 and the high-potential peritoneal dissemination gastric cancer cell line MKN-45P.
    Kurume Med. J. 58:73-79(2011)

    PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
    Kong D.-X., Yamori T.
    JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
    Bioorg. Med. Chem. 20:1947-1951(2012)

    PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
    Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
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